Molecular characterization, expression and function analysis of Epinephelus coioides MKK4 response to SGIV and Vibrio alginolyticus infection

Yu-Ling Su; Yu-Feng Jiang; He-Jia Chen; Shi Ye; Wei-Hua Zhou; Hong-Ping Liu; Jun-De Dong; Jing-Guang Wei; Qi-Wei Qin; Hongyan Sun

doi:10.1016/j.dci.2021.104020

Molecular characterization, expression and function analysis of Epinephelus coioides MKK4 response to SGIV and Vibrio alginolyticus infection

Dev Comp Immunol. 2021 Jun:119:104020. doi: 10.1016/j.dci.2021.104020. Epub 2021 Jan 19.

Authors

Affiliations

¹ Joint Laboratory of Guangdong Province and Hong Kong Regions on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou 510642, Guangdong Province, PR China.
² Department of Laboratory, Jining No.1 People's Hospital; Postdoctoral Mobile Station of Shandong University of Traditional Chinese Medicine, Shandong, 272111, PR China.
³ Hainan Key Laboratory of Tropical Marine Biotechnology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, PR China.
⁴ Joint Laboratory of Guangdong Province and Hong Kong Regions on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou 510642, Guangdong Province, PR China. Electronic address: qinqw@scau.edu.cn.
⁵ Joint Laboratory of Guangdong Province and Hong Kong Regions on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou 510642, Guangdong Province, PR China. Electronic address: hongyanlucky@scau.edu.cn.

PMID: 33476669
DOI: 10.1016/j.dci.2021.104020

Abstract

Mitogen-activated protein kinase 4 (MKK4), a member of the MAP kinase family, play important roles in response to many environmental and cellular stresses in mammals. In this study, three MKK4 subtypes, EcMKK4-1, EcMKK4-2 and EcMKK4-3, were obtained from grouper Epinephelus coioides. The open reading frame (ORF) of EcMKK4s are obtained and the EcMKK4s proteins contain highly conserved domains: a S_TKc domain, a canonical diphosphorylation group and two conserved MKKK ATP binding motifs, Asp-Phe-Gly (DFG) and Ala-Pro-Glu (APE). EcMKK4s could be found both in the cytoplasmic and nuclear. The EcMKK4s mRNA were detected in all E. coioides tissues examined with the different expression levels, and the expression were up-regulated during SGIV (Singapore grouper iridescent virus) or Vibrio alginolyticus infection. EcMKK4 could significantly reduce the activation of AP-1 reporter gene. The results suggested that EcMKK4s might play important roles in pathogen-caused inflammation.

Keywords: Apoptosis; Epinephelus coioides; MKK4; SGIV; Vibrio alginolyticus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Apoptosis / genetics
Apoptosis / immunology
Cell Line
Cloning, Molecular
Fish Diseases / immunology*
Fish Diseases / microbiology
Fish Diseases / virology
Fish Proteins / classification
Fish Proteins / genetics
Fish Proteins / immunology*
Gene Expression Profiling
Gene Expression Regulation / immunology*
Host-Pathogen Interactions / immunology
Iridovirus / immunology*
Iridovirus / physiology
MAP Kinase Kinase 4 / classification
MAP Kinase Kinase 4 / genetics
MAP Kinase Kinase 4 / immunology*
Perciformes / immunology*
Perciformes / microbiology
Perciformes / virology
Phylogeny
RNA, Messenger / genetics
RNA, Messenger / immunology
RNA, Messenger / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Sequence Homology, Amino Acid
Up-Regulation / immunology
Vibrio alginolyticus / immunology*
Vibrio alginolyticus / physiology

Substances

Fish Proteins
RNA, Messenger
MAP Kinase Kinase 4