Characterization of complete mitochondrial genome of two-spot swimming crab Charybdis bimaculata (Miers, 1886)

Nack-Keun Kim; Sapto Andriyono; Ah Ran Kim; Chung Il Lee; Hyun-Woo Kim

doi:10.1080/23802359.2018.1501292

Characterization of complete mitochondrial genome of two-spot swimming crab Charybdis bimaculata (Miers, 1886)

Mitochondrial DNA B Resour. 2018 Aug 17;3(2):900-901. doi: 10.1080/23802359.2018.1501292.

Authors

Nack-Keun Kim¹, Sapto Andriyono^{1

2}, Ah Ran Kim¹, Chung Il Lee³, Hyun-Woo Kim^{1

4}

Affiliations

¹ Interdisciplinary Program of Biomedical, Mechanical and Electrical Engineering, Pukyong National University, Busan, Republic of Korea.
² Department of Marine, Fisheries and Marine Faculty, Universitas Airlangga C Campus Jl, Mulyorejo Surabaya East Java, Indonesia.
³ Department of Marine Bioscience, Gangneung-Wonju National University, Gangneung, Republic of Korea.
⁴ Department of Marine Biology, Pukyong National University, Busan, Republic of Korea.

Abstract

The two-spot swimming crab Charybdis bimaculata (Miers, 1886) is an important decapod species in the benthic ecosystem of Korean waters. In this study, we determined its complete mitochondrial genome by the combination of NGS analysis using MiSeq platform and PCR-based cloning method. The circular mitochondrial genome of C. bimaculata was 15,714 bp in length in which the standard set of 13 protein-coding genes, 22 tRNA genes, and 2 rRNA genes were encoded. Phylogenic analysis showed that C.bimaculata is most closely related to Charybdis feriata. The complete mitogenome sequence information of C. bimaculata would provide useful data for the conservation of their population in the Pacific ocean.

Keywords: Charybdis bimaculata; Next generation sequencing; decapod; mitogenome; phylogeny.