Using ezRAD to reconstruct the complete mitochondrial genome of Porites fontanesii (Cnidaria: Scleractinia)

Tullia I Terraneo; Roberto Arrigoni; Francesca Benzoni; Zac H Forsman; Michael L Berumen

doi:10.1080/23802359.2018.1437805

Using ezRAD to reconstruct the complete mitochondrial genome of Porites fontanesii (Cnidaria: Scleractinia)

Mitochondrial DNA B Resour. 2018 Feb 9;3(1):173-174. doi: 10.1080/23802359.2018.1437805.

Authors

Tullia I Terraneo^{1

2}, Roberto Arrigoni¹, Francesca Benzoni³, Zac H Forsman⁴, Michael L Berumen¹

Affiliations

¹ Red Sea Research Center, Division of Biological and Environmental Science and Engineering, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia.
² ARC Centre of Excellence for Coral Reef Studies, James Cook University, Townsville, QLD, Australia.
³ Department of Biotechnologies and Bioscience, University of Milano-Bicocca, Milan, Italy.
⁴ Hawaii Institute of Marine Biology, Kaneohe, HI, USA.

Abstract

Corals in the genus Porites are among the major framework builders of reef structures worldwide, yet the genus has been challenging to study due to a lack of informative molecular markers. Here, we used ezRAD sequencing to reconstruct the complete mitochondrial genome of Porites fontanesii (GenBank accession number MG754069), a widespread coral species endemic to the Red Sea and Gulf of Aden. The gene arrangement of P. fontanesii did not differ from other Scleractinia and consisted of 18,658 bp, organized in 13 protein-coding genes, 2 rRNA genes, and 2 tRNA genes. This mitochondrial genome contributes essential data to work towards a better understanding of evolutionary relationships within Porites.

Keywords: Mitochondrial genome; RAD sequencing; Scleractiania.