The complete chloroplast genome sequence of Populus wilsonii and its phylogenetic analysis

Xue-Min Han; Yi-Ming Wang; Yan-Jing Liu

doi:10.1080/23802359.2017.1413291

The complete chloroplast genome sequence of Populus wilsonii and its phylogenetic analysis

Mitochondrial DNA B Resour. 2017 Dec 8;2(2):932-933. doi: 10.1080/23802359.2017.1413291.

Authors

Xue-Min Han^{1

2}, Yi-Ming Wang³, Yan-Jing Liu¹

Affiliations

¹ State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing, China.
² University of Chinese Academy of Sciences, Beijing, China.
³ College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, China.

Abstract

The complete chloroplast genome of Populus wilsonii was reconstructed by reference-based assembly using whole-genome sequencing data. The total chloroplast genome size of P. wilsonii was 158,080 bp in length, including a pair of inverted repeat regions (IRs) of 27,749 bp each, a large single-copy region (LSC) of 85,949 bp and a small single-copy region (SSC) of 16,633 bp. A total of 133 genes were predicted from the chloroplast genome, including 86 protein-coding genes, 39 tRNA genes and eight rRNA genes. Among these genes, 20 genes occurred in IRs, containing nine protein-coding genes, seven tRNA genes and four rRNA genes. The GC content of P. wilsonii chloroplast genome was 36.6%. The phylogenetic analysis with 15 other species showed that P. wilsonii was closely clustered with Populus cathayana. The complete chloroplast genome of P. wilsonii provides new insights into Populus evolutionary and genomic studies.

Keywords: Populus wilsonii; chloroplast genome; phylogenetic analysis; whole-genome sequencing.