Complete mitogenome mapping of potato late blight pathogen, Phytophthora infestans A2 mating type

Virupaksh U Patil; G Vanishree; Debasis Pattanayak; Sanjeev Sharma; Vinay Bhardwaj; B P Singh; S K Chakrabarti

doi:10.1080/23802359.2017.1280699

Complete mitogenome mapping of potato late blight pathogen, Phytophthora infestans A₂ mating type

Mitochondrial DNA B Resour. 2017 Feb 16;2(1):90-91. doi: 10.1080/23802359.2017.1280699.

Authors

Virupaksh U Patil¹, G Vanishree¹, Debasis Pattanayak², Sanjeev Sharma³, Vinay Bhardwaj¹, B P Singh⁴, S K Chakrabarti^{1

3}

Affiliations

¹ Division of Crop Improvement, Central Potato Research Institute (ICAR), Shimla, India.
² National Research Centre for Plant Biotechnology (NRCPB), New Delhi, India.
³ Division of Plant Protection, Central Potato Research Institute (ICAR), Shimla, India.
⁴ Central Potato Research Institute (ICAR), Shimla, India.

Abstract

Complete mitochondrial genome of Phytophthora infestans, A₂ mating type (MT) with a size of ≅37,767 bp was sequenced. A total of 53 protein-coding genes are predicted on both strands, including 25 tRNA, 2 rRNA, and 18 respiratory proteins. Gene order of A₂MT was consistent with that established in A₁, despite high level of polymorphism in both coding and non-coding regions. The mtDNA of A₂MT was found to have 99.5% and 99.4% homology with Ia and Ib, whereas 94.7% and 94.3% with IIa and IIb, respectively. Study of repeats revealed a dinucleotide (AT)₉ specific to A₁ and homology of cox1 gene sequence revealed the relationship among 50 Phytophthora species.

Keywords: Phytophthora; genome; haplotype mitochondria; mapping; mating type.