Complete mitochondrial genome analysis of Japanese Spanish mackerel (Scomberomorus niphonius) with phylogenetic consideration

Yan Jin; Longling Ouyang; Hongxiang Zheng; Yu Lin; Menghan Zhang; Shengfa Li; Jiahua Cheng

doi:10.1080/23802359.2017.1280700

Complete mitochondrial genome analysis of Japanese Spanish mackerel (Scomberomorus niphonius) with phylogenetic consideration

Mitochondrial DNA B Resour. 2017 Feb 2;2(1):56-57. doi: 10.1080/23802359.2017.1280700.

Authors

Yan Jin¹, Longling Ouyang¹, Hongxiang Zheng², Yu Lin¹, Menghan Zhang², Shengfa Li¹, Jiahua Cheng¹

Affiliations

¹ Key Laboratory of Marine and Estuarine Fisheries, Ministry of Agriculture of China, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai, China.
² MOE Key Laboratory of Contemporary Anthropology, School of Life Sciences, Fudan University, Shanghai, China.

Abstract

The complete mitochondrial DNA (mtDNA) of Japanese Spanish mackerel (Scomberomorus niphonius) was cloned and sequenced. The total length of the mitochondrial genome is 16,646 bp with an accession number KY228987. Thirty-seven genes are identified in total, including 13 protein-coding genes, 22 transfer RNA (tRNA) genes, 2 ribosomal RNA (rRNA) genes and a putative D-loop region. Among these genes, 9 are encoded on the light strand, while others are encoded on the heavy strand. The overall base composition of mitogenome is 28.39% for A, 16.14% for G, 26.52% for T, 28.96% for C, respectively, with a slight higher A + T content (54.91%). The phylogeny analysis based on 18 COI amino acid sequences suggested that S. niphonius and the other two species from Scomberomorus (Scombridae) formed a cluster apart from the one comprising other genus from Scombridae. The complete mitogenome may shed light on the future study of genetic mechanism of Scomberomorus niphonius.

Keywords: Scomberomorus niphonius; evolutionary relationships; gene order; genome structure; mitochondrial genome.