Pimelodus maculatus (Siluriformes, Pimelodidae): complete mtDNA sequence of an economically important fish from the São Francisco river basin

Leonardo Cardoso Resende; Anderson Oliveira do Carmo; Daniela Núñez-Rodriguez; Juliana da Silva Martins Pimentel; Alessandra Gomes Bedore; Hortênsia Gomes Leal; Evanguedes Kalapothakis

doi:10.1080/23802359.2016.1219646

Pimelodus maculatus (Siluriformes, Pimelodidae): complete mtDNA sequence of an economically important fish from the São Francisco river basin

Mitochondrial DNA B Resour. 2016 Nov 22;1(1):806-808. doi: 10.1080/23802359.2016.1219646.

Authors

Leonardo Cardoso Resende¹, Anderson Oliveira do Carmo¹, Daniela Núñez-Rodriguez¹, Juliana da Silva Martins Pimentel², Alessandra Gomes Bedore², Hortênsia Gomes Leal², Evanguedes Kalapothakis²

Affiliations

¹ Biologia Geral, Universidade Federal de Minas Gerais Instituto de Ciencias Biologicas, Belo Horizonte, Brazil.
² Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.

Abstract

Pimelodus maculatus is an important commercial fish found in the São Francisco and Paraná river basins. NGS was used to sequence the mtDNA of P. maculatus. The mtDNA was annotated and aligned with that of 25 other fish species to enable phylogenetic analysis. The complete mtDNA molecule had 16,561 bp and its GC content was 43.7%; the structure was similar to that of other vertebrates: 2 rRNA, 22 tRNA, 13 protein-coding genes, and a D-loop region containing 914 bp. Phylogenetic analysis yielded a tree with a high bootstrap coefficient that was coherent with the actual phylogeny of the species.

Keywords: Complete mtDNA; Pimelodus maculatus; next-generation sequencing; ‘mandi’; ‘yellow-mandi catfish’.