Sequence and organization of the complete mitochondrial genome of the blackfly Simulium variegatum (Diptera: Simuliidae)

John C Day; Hyun S Gweon; Rory J Post

doi:10.1080/23802359.2016.1209091

Sequence and organization of the complete mitochondrial genome of the blackfly Simulium variegatum (Diptera: Simuliidae)

Mitochondrial DNA B Resour. 2016 Nov 22;1(1):799-801. doi: 10.1080/23802359.2016.1209091.

Authors

John C Day¹, Hyun S Gweon¹, Rory J Post^{2

3}

Affiliations

¹ Centre for Ecology and Hydrology (CEH) Wallingford, Wallingford, Oxfordshire, UK.
² School of Natural Sciences and Psychology, Liverpool John Moores University, Liverpool, UK.
³ Disease Control Department, London School of Hygiene & Tropical Medicine, London, UK.

Abstract

The complete mitochondrial genome of the European blackfly, Simulium variegatum Meigen, 1818 was sequenced using a combined Illumina and Sanger sequencing approach. Using the known sequence of Chironomus tepperi Skuse, 1889 (Chironomidae) homologous NGS reads were identified and assembled. The genome is 15,367 bp in length and includes 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes and a control region. Gene order resembles that of the ancestral dipteran gene arrangement. The base composition of the genome is A (37.6%), T (35.3%), C (15.8%) and G (11.3%). The control region between 12S rRNA and tRNAIle is composed of 362 bp with no obvious repetitive motifs.

Keywords: Blackfly; Diptera; Simuliidae; Simulium variegatum; mitochondrial genome.