The mitochondrial genome of the western bean cutworm, Striacosta albicosta (Lepidoptera: Noctuidae)

Brad S Coates; Craig A Abel

doi:10.1080/23802359.2016.1192499

The mitochondrial genome of the western bean cutworm, Striacosta albicosta (Lepidoptera: Noctuidae)

Mitochondrial DNA B Resour. 2016 Jul 10;1(1):487-488. doi: 10.1080/23802359.2016.1192499.

Authors

Brad S Coates^{1

2}, Craig A Abel^{1

2}

Affiliations

¹ United States Department of Agriculture, Agricultural Research Service, Corn Insects & Crop Genetics Research Unit, Ames, IA, USA.
² Department of Entomology, Iowa State University, Ames, IA, USA.

Abstract

The complete 15,553 bp mitochondrial genome of the western bean cutworm, Stricosta albicosta, (Lepidoptera: Noctuidae) was assembled from paired end Illumina HiSeq2500 read data. Annotation showed 13 predicted protein coding genes (PCGs), 22 tRNAs and 2 rRNAs have an order and orientation typical of insect mitochondrial genomes, and the derived rearrangement of tRNA-Met, -Ile and -Gln upstream of nad2 as in other Lepidoptera. A 79.3% A + T content resulted in a bias for codons with A or T in the 3rd position, and prevalence of synonymous substitutions suggest the effects of purifying selection on the mitochondrial genome sequence. Two microsatellite repeat motifs, (CA)₁₀(AT)₁₉ and (AT)₁₂, are respectively located in intergenic spaces between tRNA-Glu and -Phe and tRNA-Leu and 16S rRNA. Mitochondrial phylogenomics was able to resolve sub-families within the Noctuidae, and suggest analogous analyses may be applicable across other lepidopteran Families.

Keywords: Invasive species; next generation sequencing; pest insect.