Rice Haploid Inducer Development by Genome Editing

Methods Mol Biol. 2021:2238:221-230. doi: 10.1007/978-1-0716-1068-8_14.

Abstract

The current method to induce haploids in rice is anther culture, which is time-consuming and labor intensive and only works for some varieties. Here we describe a seed-based haploid induction system created by CRISPR/Cas9 technology. By editing OsMATL, we generate rice haploid inducer lines with a 2-5% haploid induction rate in different germplasms.

Keywords: CRISPR/Cas9; Crossing; Genome editing; Haploid inducer; OsMATL; Pollination; Rice.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • CRISPR-Cas Systems*
  • Gene Editing*
  • Gene Transfer Techniques
  • Genetic Vectors / genetics
  • Genome, Plant*
  • Haploidy*
  • Oryza / genetics
  • Oryza / growth & development*
  • Plant Breeding
  • Plant Proteins / genetics*

Substances

  • Plant Proteins