Abstract
Thioflavin-T is used to image amyloid aggregates because of the excellent turn-on fluorescence properties, but binding affinities are low. By mounting multiple dye units on the surface of a vesicle, the binding affinity for α-synuclein fibrils is increased by three orders of magnitude, and the optical response is increased. Cooperative interactions of the dye headgroup and lipid with the protein provide a general strategy for the construction of multivalent amyloid probes based on vesicles.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amyloid / chemistry*
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Amyloid / metabolism
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Amyloid beta-Peptides / chemistry
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Amyloid beta-Peptides / metabolism*
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Benzothiazoles / chemistry*
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Benzothiazoles / metabolism
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Fluorescence
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Fluorescent Dyes / chemistry*
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Liposomes
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Molecular Structure
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Protein Aggregates
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Protein Binding
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alpha-Synuclein / chemistry*
Substances
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Amyloid
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Amyloid beta-Peptides
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Benzothiazoles
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Fluorescent Dyes
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Liposomes
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Protein Aggregates
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alpha-Synuclein
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thioflavin T