The effect of concentration, temperature, and the addition of graphene oxide (GO) nanosheets on the rheological and dielectric behavior of chitosan (CS) solutions, which influences the formation of the blend materials for various applications including 3D printing and packaging, was studied. Among tested acid solutions, the rheological behavior of 1% CS in acetic and lactic acid solutions was found to be similar, whereas the hydrochloric acid solution showed an abnormal drop in the dynamic moduli. Oscillatory rheology confirmed a distinct gel point for the CS solutions at below 10 °C. Both the G' and G″ of the solutions increased with the loading concentrations of GO between 0.5 and 1%, and it marginally dropped at the loading concentration of 2%, which is consistent with AFM observation. The steady-shear flow data fitted the Carreau model. Dielectric property measurement further confirmed that both the dielectric constant, ε' and the loss factor, ε″ for the CS in hydrochloric acid solutions behaved differently from others. Addition of GO significantly improved both ε' and ε″, indicating an improvement in the dielectric properties of CS/GO solutions. The dispersion of GO into the CS matrix was assessed by measuring XRD, FTIR, and microscopy of the film prepared from the solutions. Furthermore, the inclusion of GO into CS solution containing pluronic F127 (F127) base for potential 3D printing application showed positive results in terms of the printing accuracy and shape fidelity of the printed objects (films and scaffolds). The optimized composition with homogeneous particle distribution indicated that up to ∼50 mg/mL GO concentration (w/v of F127 base) was suitable to print both films and scaffolds for potential biomedical applications.
Keywords: 3D printing; Chitosan solution; Dielectric constant; TGA; Zero shear rate viscosity; bioinks.