Earlier diagnoses of acute leukemia by a sandwich type of electrochemical aptasensor based on copper sulfide-graphene composite

Abstract

Due to high affinity and specificity of aptamers, they are widely considered for construction of aptasensor to specific recognizing of analytes in biological complex matrix. So, in this work we design a high selective and sensitive aptasensor for leukemia cancer cells (CCRF-CEM) via superior catalytic effect of copper sulfide-graphene (CuS-GR) nanocomposite as label and Au-GR nanocomposite as sensing platform. The CuS-GR nano-composite (label component) is CuS nanoparticles that wrapping on graphene sheets. Its catalytic activity (CuS-GR) increases the current of sensor in parallel with adding of CCRF-CEM and provide sensitive detection of analytes. The detailed of signal amplification and effect on the aptasensor performance completely discussed. This sensor has a linear range of 50-1 × 10⁶ cell mL^-1, with a limit of detection of 18 cell mL^-1. Also, the developed aptasensor has a significance specificity, high sensitivity and accuracy. It was used for the identification of CCRF-CEM cells in blood samples.

Keywords: Aptasensor; Catechol; Copper sulfide; Leukemia cancer cells; Screen-printed electrode.