Generation of a DKK1 homozygous knockout human embryonic stem cell line WAe001-A-21 using CRISPR/Cas9

Stem Cell Res. 2021 Mar:51:102161. doi: 10.1016/j.scr.2021.102161. Epub 2021 Jan 8.

Abstract

Dickkopf1 (DKK1) is a secreted inhibitor for the Wnt signalling, which is involved in cell proliferation, tissue regeneration and embryonic development. Using CRISPR/Cas9 editing, we established a homozygous mutant DKK1 human embryonic stem cell line (WAe001-A-21). It has a 41 bp deletion in exon 2 of DKK1, leading to its coding frame shift. The WAe001-A-21 cell line maintains a normal karyotype, pluripotency markers, typical stem cell morphology and the ability to differentiate into three germ layers.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • CRISPR-Cas Systems / genetics
  • Cell Line
  • Clustered Regularly Interspaced Short Palindromic Repeats
  • Embryonic Stem Cells
  • Human Embryonic Stem Cells*
  • Humans
  • Intercellular Signaling Peptides and Proteins / genetics

Substances

  • DKK1 protein, human
  • Intercellular Signaling Peptides and Proteins