Hamstring muscles rupture under traction, peeling and shear lap tests: A biomechanical study in rabbits

Abstract

Lesions of the Musculotendinous Unit (MTU, i.e. tendon, myotendinous junction, muscle, aponeurosis and myoaponeurotic junction) are a common injury and a leading cause of functional impairment, long-term pain, and/or physical disability worldwide. Though a large effort has been devoted to macroscopic failure evaluation, these injuries suffer from a lack of knowledge of the underlying tissue-scale micro-mechanisms triggering such lesions. More specifically, there is a strong need for experimental data to better understand and quantify damage initiation and propagation on MTUs. The present study presents original experimental data on muscle tissue extracted from the hamstring muscle group of rabbits under relevant mechanical solicitations up to rupture, revealing elementary micro-mechanisms and providing quantified values of elastic properties as well as initiation stress and energy release rate. More specifically, tensile, peeling and shear lap tests were performed to explore cohesion of muscle tissue along the fibre direction or across fibres (mode I) and in shear (mode II), as well as at the muscle/tendon interface. We show that muscle tissue is weaker in shear than tension (p-value < 0.01) and that the Biceps Femoris had the lowest energy release rate as calculated from mode I peeling tests (G = 0.23 ± 0.16 N/mm) compared to the Semi-Membranous (G = 0.53 ± 0.08 N/mm) and the Semi-Tendinous (0.45 ± 0.20 N/mm), and that this energy is the lowest at the musculotendinous junction. Our study suggests a preferred damage initiation mechanism based on fibre decohesion in mode I or II and provides quantitative data to model these phenomena. Results also suggest that the Biceps Femoris and more precisely its musculotendinous junction could be the weakest point of the hamstring group. These findings could be used as a basis to develop mechanical models (e.g. finite element) to better understand and predict the onset of hamstring lesions and help in preventing such events.

Keywords: Energy release rate; Hamstring muscles; Muscle injury; Peeling test; Rupture test; Shear lap test.