Preparation and culture of bone marrow-derived macrophages from mice for functional analysis

Gotaro Toda; Toshimasa Yamauchi; Takashi Kadowaki; Kohjiro Ueki

doi:10.1016/j.xpro.2020.100246

Preparation and culture of bone marrow-derived macrophages from mice for functional analysis

STAR Protoc. 2020 Dec 31;2(1):100246. doi: 10.1016/j.xpro.2020.100246. eCollection 2021 Mar 19.

Authors

Gotaro Toda¹, Toshimasa Yamauchi¹, Takashi Kadowaki², Kohjiro Ueki³

Affiliations

¹ Department of Diabetes and Metabolic Diseases, Graduate School of Medicine, the University of Tokyo, Tokyo, Japan.
² Toranomon Hospital, Tokyo, Japan.
³ Department of Molecular Diabetic Medicine, Diabetes Research Center, Research Institute, National Center for Global Health and Medicine, Tokyo, Japan.

Abstract

The assessment of macrophage function has been a topic of intense discussion due to multiple subtypes. This protocol describes the collection of bone marrow cells from the femur and tibia of mice, differentiation into bone marrow-derived macrophages (BMDM cells), and sampling from cultures. This protocol focuses on the efficient preparation of BMDM cells, providing a way to assess the function of macrophages. For complete details on the use and execution of this protocol, please refer to Toda et al. (2020).

Keywords: Cell Differentiation; Cell isolation; Immunology; Model Organisms.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bone Marrow Cells / cytology
Bone Marrow Cells / metabolism*
Cell Culture Techniques*
Cell Separation*
Cells, Cultured
Macrophages / cytology
Macrophages / metabolism*
Mice