Characterization of the complete chloroplast genome of a Chinese endangered species Cymbidium mannii

Yuying Wang; Longjie Cheng; Yiran Zhao; Yefang Li; Fengmei He; Zhilin Li

doi:10.1080/23802359.2020.1810159

Characterization of the complete chloroplast genome of a Chinese endangered species Cymbidium mannii

Mitochondrial DNA B Resour. 2020 Aug 25;5(3):3199-3200. doi: 10.1080/23802359.2020.1810159.

Authors

Yuying Wang¹, Longjie Cheng¹, Yiran Zhao¹, Yefang Li¹, Fengmei He¹, Zhilin Li¹

Affiliation

¹ College of Horticulture and Landscape, Yunnan Agricultural University, Kunming, China.

Abstract

Cymbidium mannii is an endangered species belonging to the first ranking in protection category in China with important ornamental value and breeding value. This study used Illumina high-throughput sequencing technologies to sequence and analyze the complete chloroplast genome of C. mannii. The genome features of C. mannii and the phylogenetic relationships among Orchidaceae species were reported and established. The complete chloroplast genome is 152,544 bp in length, consisting of a pair of inverse duplication regions 25,357 bp, a large single-copy region 86,014 bp and a small single-copy region 15,816 bp. The entire genome contains 74 mRNA genes, 30 tRNA genes and 4 rRNA genes. The phylogenetic tree of 23 Orchidaceae species revealed C. mannii is more closely related to Cymbidium aloifolium.

Keywords: Cymbidium mannii; chloroplast genome; endangered species; phylogenetic analysis.

Grants and funding

This work was supported by the Yunnan Province Major Project of China [grant number 2018BB010], Yunnan Province Basic Research Project of China [grant number 2018FB070], Open Fund project of Yunnan Key Laboratory of Flower Breeding of China [grant number FKL-201602], Key Laboratory Project of Education Department of Yunnan Province of China and Innovation Team of Universities Project of Education Department of Yunnan Province of China.