Potassium superoxide as an intricate source of superoxide anion. Elucidating the composition of its samples in dimethyl sulfoxide by reactions with (5,10,15,20-tetraphenylporphinato)manganese(III) chloride and curcumin

Yurii B Tsaplev; Aleksei V Trofimov

doi:10.1016/j.saa.2021.119425

Potassium superoxide as an intricate source of superoxide anion. Elucidating the composition of its samples in dimethyl sulfoxide by reactions with (5,10,15,20-tetraphenylporphinato)manganese(III) chloride and curcumin

Spectrochim Acta A Mol Biomol Spectrosc. 2021 Apr 15:251:119425. doi: 10.1016/j.saa.2021.119425. Epub 2021 Jan 5.

Authors

Yurii B Tsaplev¹, Aleksei V Trofimov²

Affiliations

¹ Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, ul. Kosygina 4, 119334 Moscow, Russian Federation. Electronic address: tsap_04@mail.ru.
² Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, ul. Kosygina 4, 119334 Moscow, Russian Federation; Moscow Institute of Physics and Technology (National Research University), Institutskii per. 9, 141701 Dolgoprudny, Moscow Region, Russian Federation. Electronic address: avt_2003@mail.ru.

PMID: 33453597
DOI: 10.1016/j.saa.2021.119425

Abstract

The fundamentals and approbation of the new spectral assay for superoxide anion and KOH (inevitable impurity in KO₂ preparations) in a dimethyl sulfoxide (DMSO) solution of potassium superoxide (KO₂), a widely used source of superoxide anion in diverse chemical and biochemical studies, are disclosed. To assess the concentration of superoxide anion and KOH, it was proposed to use (5,10,15,20-tetraphenylporphinato)manganese(III) chloride as a spectrophotometric probe for superoxide anion and curcumin for the determination of KOH based on recently acquired spectral properties of curcumin in alkaline DMSO. We have found that the prepared solutions generate superoxide anion in the concentration of 1.7 mM and contain KOH in the concentration of 3 mM, which is close to the saturated concentration in anhydrous DMSO.

Keywords: Curcumin; Mn(III)TPP; Potassium superoxide; Spectral measurements; Superoxide anion.