Key factors governing the performance and microbial community of one-stage partial nitritation and anammox system with bio-carriers and airlift circulation

Hong Chen; Hong Wang; Guanlong Yu; Ying Xiong; Haipeng Wu; Min Yang; Rong Chen; Enzhe Yang; Changbo Jiang; Yu-You Li

doi:10.1016/j.biortech.2021.124668

Key factors governing the performance and microbial community of one-stage partial nitritation and anammox system with bio-carriers and airlift circulation

Bioresour Technol. 2021 Mar:324:124668. doi: 10.1016/j.biortech.2021.124668. Epub 2021 Jan 6.

Authors

Hong Chen¹, Hong Wang², Guanlong Yu², Ying Xiong², Haipeng Wu², Min Yang³, Rong Chen⁴, Enzhe Yang², Changbo Jiang², Yu-You Li⁵

Affiliations

¹ Key Laboratory of Water-Sediment Sciences and Water Disaster Prevention of Hunan Province, School of Hydraulic Engineering, Changsha University of Science & Technology, Changsha 410004, China; Laboratory of Environmental Protection Engineering, Department of Civil and Environmental Engineering, Graduate School of Engineering, Tohoku University, 6-6-06 Aza-Aoba, Aramaki, Aoba Ward, Sendai, Miyagi 980-8579, Japan.
² Key Laboratory of Water-Sediment Sciences and Water Disaster Prevention of Hunan Province, School of Hydraulic Engineering, Changsha University of Science & Technology, Changsha 410004, China.
³ School of Chemistry and Food Engineering, Changsha University of Science & Technology, Changsha 410004, China.
⁴ Laboratory of Environmental Protection Engineering, Department of Civil and Environmental Engineering, Graduate School of Engineering, Tohoku University, 6-6-06 Aza-Aoba, Aramaki, Aoba Ward, Sendai, Miyagi 980-8579, Japan; International S&T Cooperation Center for Urban Alternative Water Resources Development, Key Lab of Environmental Engineering, Shanxi Province, Xi'an University of Architecture and Technology, Xi'an 710055, China.
⁵ Laboratory of Environmental Protection Engineering, Department of Civil and Environmental Engineering, Graduate School of Engineering, Tohoku University, 6-6-06 Aza-Aoba, Aramaki, Aoba Ward, Sendai, Miyagi 980-8579, Japan; Graduate School of Environmental Studies, Tohoku University, 6-6-06 Aza-Aoba, Aramaki, Aoba Ward, Sendai, Miyagi 980-8579, Japan. Electronic address: gyokuyu.ri.a5@tohoku.ac.jp.

PMID: 33453520
DOI: 10.1016/j.biortech.2021.124668

Abstract

A one-stage airlift internal circulation biofilm reactor was continuously operated for 668 days to treat 50 mg/L of ammonia wastewater to pursue the long-term stability of partial nitritation and anammox (PNA) process. The operational performance and microbial community structure of the biofilm and the flocs were investigated. A nitrogen removal efficiency (NRE) of 70% was obtained successfully at a dissolved oxygen (DO) of 0.05-0.15 mg/L by regulating aeration rate. The microbial analysis indicated Candidatus Brocadia (29.5%) and Nitrosomonas (6.8%) were dominant in both biofilms and flocs. It was found that DO control and aeration rate were the key factors in performance stability, and a stable performance could be recovered and maintained under oxygen-limiting conditions. Further, the achievement of activated ammonia oxidation bacteria (AOB), dominated anammox bacteria (AMX), suppressed NOB, and controlled heterotrophic bacteria (HB) in the biofilms played a major role in the long-term stable operation.

Keywords: Airlift; Bio-carriers; Brocadia; Key factors; Partial nitritation and anammox (PNA).

MeSH terms

Ammonia
Ammonium Compounds*
Biofilms
Bioreactors
Microbiota*
Nitrogen
Oxidation-Reduction

Substances

Ammonium Compounds
Ammonia
Nitrogen