Toxic effect of p-chloroaniline and butyl acrylateon Nannochloropsis oculata based on water samples from two sea areas

Shouxiang Wei; Jingjing Miao; Yuhan Li; Yusong Li; Xiufen Wang; Luqing Pan; Yun Li; Jiangyue Wu; Yufei Lin

doi:10.1016/j.etap.2021.103582

Toxic effect of p-chloroaniline and butyl acrylateon Nannochloropsis oculata based on water samples from two sea areas

Environ Toxicol Pharmacol. 2021 Apr:83:103582. doi: 10.1016/j.etap.2021.103582. Epub 2021 Jan 11.

Authors

Shouxiang Wei¹, Jingjing Miao¹, Yuhan Li¹, Yusong Li², Xiufen Wang¹, Luqing Pan¹, Yun Li³, Jiangyue Wu⁴, Yufei Lin⁴

Affiliations

¹ The Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, Qingdao, Shangdong, China.
² Faculty of Science, Western University, London, Ontario, N6A 5B7, Canada.
³ The Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, Qingdao, Shangdong, China. Electronic address: 15318790309@163.com.
⁴ National Marine Hazard Mitigation Service, State Ocean Administration, Beijing, 100194, China.

PMID: 33444758
DOI: 10.1016/j.etap.2021.103582

Abstract

To compare the influence of water samples collected from various areas on toxic effect of HNS, we examined the toxic effect of two commonly found HNS: p-chloroaniline and butyl acrylate, on Nannochloropsis oculata cultured in seawater collected from Laizhou bay and Jiaozhou bay (China). The results showed that both p-chloroaniline and butyl acrylate had significant toxic effect on N. oculata cultured in both water samples. P-chloroaniline inhibited its net oxygenation rate and oxygen consumption rate. Butyl acrylate inhibited the net oxygenation rate whereas significantly stimulated oxygen consumption rate. Performance of N. oculata changed between two water samples under same level of p-chloroaniline and butyl acrylate. The net oxygenation rate of N. oculata cultured in the seawater from the Jiaozhou bay increased by 11.60 %, the oxygen consumption rate increased by 26.91 %, algae cell growth decreased by 16.83 %, compared to those from Laizhou bay. The Fv/Fm of N. oculata cultured in Jiaozhou bay was more significantly inhibited at 0.5 and 2.0 mg L^-1 p-chloroaniline, while it was significantly inhibited at 5. 0 mg L^-1 of butyl acrylate, compared to those from Laizhou bay. Moreover, the toxic effect of both HNS on net oxygenation rate and oxygen consumption rate were significantly attenuated as the concentration increased. The growth inhibition of microalgae cultured in two seawater samples was more evident at 0.5 and 5.0 mg L^-1 p-chloroaniline than at 2.0 mg L^-1 p-chloroaniline,and the growth inhibition of microalgae cultured in two seawater samples was more evident at 2.0 and 5.0 mg L^-1 butyl acrylate than at 0.5 mg L^-1 butyl acrylate. These results indicated that toxic effect of p-chloroaniline and butyl acrylate on the growth of N. oculata was influenced by the pollutants in the two water samples. Consequently, a corresponding research on water sample is required in advance to increase accuracy of future ecological risk assessment of HNS.

Keywords: Butyl acrylate; Comparative analysis; HNS; Jiaozhou bay; Laizhou bay; N. oculata; P-chloroaniline.

MeSH terms

Acrylates / toxicity*
Aniline Compounds / toxicity*
Microalgae / drug effects*
Microalgae / growth & development
Seawater
Stramenopiles / drug effects*
Stramenopiles / growth & development
Water Pollutants, Chemical / toxicity*

Substances

Acrylates
Aniline Compounds
Water Pollutants, Chemical
n-butyl acrylate
4-chloroaniline