Assembly of Dynamic Supramolecular Polymers on a DNA Origami Platform

Jurgen Schill; Bas J H M Rosier; Berta Gumí Audenis; Eva Magdalena Estirado; Tom F A de Greef; Luc Brunsveld

doi:10.1002/anie.202016244

Assembly of Dynamic Supramolecular Polymers on a DNA Origami Platform

Angew Chem Int Ed Engl. 2021 Mar 29;60(14):7612-7616. doi: 10.1002/anie.202016244. Epub 2021 Feb 26.

Authors

Jurgen Schill^{1

2}, Bas J H M Rosier^{1

2}, Berta Gumí Audenis^{1

3}, Eva Magdalena Estirado^{1

2}, Tom F A de Greef^{1

2

4

5}, Luc Brunsveld^{1

2}

Affiliations

¹ Institute for Complex Molecular Systems, Eindhoven University of Technology, P.O. Box 513, 5600, MB, Eindhoven, The Netherlands.
² Laboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of Technology, The Netherlands.
³ Laboratory of Self-Organising Soft Matter and Laboratory of Macromolecular and Organic Chemistry, Department of Chemical Engineering and Chemistry, Eindhoven University of Technology, The Netherlands.
⁴ Computational Biology group, Department of Biomedical Engineering, Eindhoven University of Technology, The Netherlands.
⁵ Institute for Molecules and Materials, Faculty of Science, Radboud University, Heyendaalseweg 135, 6525, AJ, Nijmegen, The Netherlands.

Abstract

Biological processes rely on transient interactions that govern assembly of biomolecules into higher order, multi-component systems. A synthetic platform for the dynamic assembly of multicomponent complexes would provide novel entries to study and modulate the assembly of artificial systems into higher order topologies. Here, we establish a hybrid DNA origami-based approach as an assembly platform that enables dynamic templating of supramolecular architectures. It entails the site-selective recruitment of supramolecular polymers to the platform with preservation of the intrinsic dynamics and reversibility of the assembly process. The composition of the supramolecular assembly on the platform can be tuned dynamically, allowing for monomer rearrangement and inclusion of molecular cargo. This work should aid the study of supramolecular structures in their native environment in real-time and incites new strategies for controlled multicomponent self-assembly of synthetic building blocks.

Keywords: multicomponent assembly; polymers; self-assembly; supramolecular synthesis.

Publication types

Research Support, Non-U.S. Gov't