Proline-Modified UIO-66 as Nanocarriers to Enhance Candida rugosa Lipase Catalytic Activity and Stability for Electrochemical Detection of Nitrofen

Yongfa Cheng; Oi-Ming Lai; Chin-Ping Tan; Worawan Panpipat; Ling-Zhi Cheong; Cai Shen

doi:10.1021/acsami.0c17134

Proline-Modified UIO-66 as Nanocarriers to Enhance Candida rugosa Lipase Catalytic Activity and Stability for Electrochemical Detection of Nitrofen

ACS Appl Mater Interfaces. 2021 Jan 27;13(3):4146-4155. doi: 10.1021/acsami.0c17134. Epub 2021 Jan 13.

Authors

Yongfa Cheng^{1

2}, Oi-Ming Lai^{2

3

4}, Chin-Ping Tan^{2

5}, Worawan Panpipat⁶, Ling-Zhi Cheong^{1

2}, Cai Shen⁷

Affiliations

¹ Department of Food Science and Engineering, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315211, China.
² Zhejiang-Malaysia Joint Research Laboratory for Agricultural Product Processing and Nutrition, Ningbo 315211, China.
³ Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
⁴ Institute of Bioscience, University Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
⁵ Department of Food Technology, Faculty of Food Science and Technology, University Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
⁶ Food Technology and Innovation Research Center of Excellence, Department of Agro-Industry, School of Agricultural Technology, Walailak University, Thasala, Nakhon Si Thammarat 80161, Thailand.
⁷ Institute of Materials Technology & Engineering, Chinese Academy of Sciences, 1219 Zhongguan Road, Ningbo 315201, China.

PMID: 33440928
DOI: 10.1021/acsami.0c17134

Abstract

Immobilization can be used to improve the stability of lipases and enhances lipase recovery and reusability, which increases its commercial value and industrial applications. Nevertheless, immobilization frequently causes conformational changes of the lipases, which decrease lipase catalytic activity. in the present work, we synthesized UIO-66 and grafted UIO-66 crystals with proline for immobilization of Candida rugosa lipase (CRL). As indicated by steady-state fluorescence microscopy, grafting of proline onto UIO-66 crystals induced beneficial conformational change in CRL. CRL immobilized on UIO-66/Pro (CRL@UIO-66/Pro) demonstrated higher enzyme activity and better recyclability than that immobilized on UIO-66 (CRL@UIO-66) in both hydrolysis (CRL@UIO-66/Pro: 0.34 U; CRL@UIO-66: 0.15 U) and transesterification (CRL@UIO-66/Pro: 0.93 U; CRL@UIO-66: 0.25 U) reactions. The higher values of k_cat and k_cat/K_m of CRL@UIO-66/Pro also showed that it had better catalytic efficiency as compared to CRL@UIO-66. It is also worth noting that CRL@UIO-66/Pro (0.93 U) demonstrated a much higher transesterification activity as compared to free CRL (0.11 U), indicating that UIO-66/Pro has increased the solvent stability of CRL. Both CRL@UIO-66 and CRL@UIO-66/Pro were also used for the fabrication of biosensors for nitrofen with a wide linear range (0-100 μM), lower limit of detection, and good recovery rate.

Keywords: Candida rugosa lipase; UIO-66; electrochemical detection; immobilization; nitrofen; proline.

MeSH terms

Biosensing Techniques / methods
Enzyme Stability
Enzymes, Immobilized / chemistry
Limit of Detection
Lipase / chemistry*
Models, Molecular
Organometallic Compounds / chemistry*
Pesticides / analysis*
Phenyl Ethers / analysis*
Phthalic Acids / chemistry*
Proline / analogs & derivatives*
Saccharomycetales / enzymology*

Substances

Enzymes, Immobilized
Organometallic Compounds
Pesticides
Phenyl Ethers
Phthalic Acids
UiO-66
Proline
Lipase
nitrofen

Supplementary concepts

Diutina rugosa