Molecular biology interventions for activity improvement and production of industrial enzymes

Shashi Kant Bhatia; Narisetty Vivek; Vinod Kumar; Neha Chandel; Meenu Thakur; Dinesh Kumar; Yung-Hun Yang; Arivalagan Pugazendhi; Gopalakrishnan Kumar

doi:10.1016/j.biortech.2020.124596

Molecular biology interventions for activity improvement and production of industrial enzymes

Bioresour Technol. 2021 Mar:324:124596. doi: 10.1016/j.biortech.2020.124596. Epub 2020 Dec 24.

Authors

Shashi Kant Bhatia¹, Narisetty Vivek², Vinod Kumar², Neha Chandel³, Meenu Thakur⁴, Dinesh Kumar⁵, Yung-Hun Yang¹, Arivalagan Pugazendhi⁶, Gopalakrishnan Kumar⁷

Affiliations

¹ Department of Biological Engineering, College of Engineering, Konkuk University, Seoul 05029, Republic of Korea; Institute for Ubiquitous Information Technology and Application, Konkuk University, Seoul 05029, Republic of Korea.
² Centre for Climate and Environmental Protection, School of Water, Energy and Environment, Cranfield University, Cranfield MK43 0AL, UK.
³ School of Medical and Allied Sciences, GD Goenka University, Gurugram 122103, Haryana, India.
⁴ Department of Biotechnology, Shoolini Institute of Life Sciences and Business Management, Solan 173212, Himachal Pradesh, India.
⁵ School of Bioengineering & Food Technology, Shoolini University of Biotechnology and Management Sciences, Solan 173229, Himachal Pradesh, India.
⁶ Innovative Green Product Synthesis and Renewable Environment Development Research Group, Faculty of Environment and Labour Safety, Ton Duc Thang University, Ho ChiMinh City, Viet Nam.
⁷ Institute of Chemistry, Bioscience and Environmental Engineering, Faculty of Science and Technology, University of Stavanger, Box 8600 Forus, 4036 Stavanger, Norway; School of Civil and Environmental Engineering, Yonsei University, Seoul 03722, Republic of Korea. Electronic address: gopalakrishnan.kumar@uis.no.

PMID: 33440311
DOI: 10.1016/j.biortech.2020.124596

Abstract

Metagenomics and directed evolution technology have brought a revolution in search of novel enzymes from extreme environment and improvement of existing enzymes and tuning them towards certain desired properties. Using advanced tools of molecular biology i.e. next generation sequencing, site directed mutagenesis, fusion protein, surface display, etc. now researchers can engineer enzymes for improved activity, stability, and substrate specificity to meet the industrial demand. Although many enzymatic processes have been developed up to industrial scale, still there is a need to overcome limitations of maintaining activity during the catalytic process. In this article recent developments in enzymes industrial applications and advancements in metabolic engineering approaches to improve enzymes efficacy and production are reviewed.

Keywords: Cosmetic; Directed evolution; Enzyme; Fusion protein; Metagenomics; Pharmaceutical.

Publication types

Review

MeSH terms

Catalysis
Directed Molecular Evolution*
Enzymes / genetics
Enzymes / metabolism
Industry*
Molecular Biology
Protein Engineering
Substrate Specificity

Substances

Enzymes