Effect of maduramicin on crayfish (Procambius clarkii): Hematological parameters, oxidative stress, histopathological changes and stress response

Xiuge Gao; Xiaoxiao Liu; Xinhao Song; Pei Teng; Hui Ji; Lin Peng; Yawei Qiu; Dawei Guo; Shanxiang Jiang

doi:10.1016/j.ecoenv.2021.111896

Effect of maduramicin on crayfish (Procambius clarkii): Hematological parameters, oxidative stress, histopathological changes and stress response

Ecotoxicol Environ Saf. 2021 Mar 15:211:111896. doi: 10.1016/j.ecoenv.2021.111896. Epub 2021 Jan 10.

Authors

Xiuge Gao¹, Xiaoxiao Liu¹, Xinhao Song¹, Pei Teng¹, Hui Ji¹, Lin Peng¹, Yawei Qiu¹, Dawei Guo¹, Shanxiang Jiang²

Affiliations

¹ Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, PR China; Laboratory of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, PR China.
² Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, PR China; Laboratory of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Nanjing Agricultural University, 1 Weigang, Nanjing 210095, PR China. Electronic address: nauvy@sina.com.

PMID: 33440267
DOI: 10.1016/j.ecoenv.2021.111896

Abstract

Maduramicin, an extensively used anticoccidial drug, has been introduced into environment due to poorly absorbed in the intestine of broiler chicken. To understand the potential ecological toxicity of maduramicin on aquatic organisms, acute and subacute toxicity, hemolymph biochemistry, histopathology and the expressions of drug metabolism and stress response genes of crayfish (Procambius clarkii) were investigated in this study. For the first time, the 96 h median lethal concentration (LC₅₀) of maduramicin on crayfish was 67.03 mgL^-1 with a 95% confidence interval (54.06-81.32 mgL^-1). Then, the crayfish were exposed to 0.7 mgL^-1 (1/100 LC₅₀), 3.5 mgL^-1 (1/20 LC₅₀) and 7.0 mgL^-1 (1/10 LC₅₀) maduramicin for 28 days. Maduramicin significantly altered biochemical parameters including AST, ALT, CK, LDH and ALP of hemolymph in crayfish at several time points. The activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) of crayfish gills, hepatopancreas and abdominal muscle were significantly decreased or elevated by different concentrations of maduramicin treatment at varying time points. Furthermore, histopathological damage of crayfish gills, hepatopancreas and abdominal muscle were observed in a concentration-dependent manner. The expressions of metabolic and stress response genes (CYP450, GST, COX1, COX2, HSP70 and MT) in hepatopancreas of crayfish were significantly up-regulated by maduramicin (7.0 mgL^-1) treatment for 8 h to 7 d, and returned to normal levels after the removal of maduramicin for 3-7 days. In conclusion, our findings demonstrated that environmental exposure of maduramicin threaten to the health of crayfish living in the areas nearby livestock farms or pharmaceutical factory. Crayfish exhibited resistance to the stress of maduramicin via activating drug metabolite and detoxification pathways.

Keywords: Acute toxicity; Crayfish; Maduramicin; Oxidative stress; Stress response; Subacute toxicity.

MeSH terms

Animals
Anti-Bacterial Agents / toxicity*
Aquatic Organisms / metabolism
Astacoidea / drug effects
Astacoidea / physiology*
Catalase / metabolism
Gills / drug effects
Glutathione Peroxidase / metabolism
Hemolymph / metabolism
Hepatopancreas / drug effects
Inactivation, Metabolic
Lactones / toxicity*
Oxidative Stress / drug effects
Seafood
Superoxide Dismutase / metabolism
Water Pollutants, Chemical / toxicity*

Substances

Anti-Bacterial Agents
Lactones
Water Pollutants, Chemical
maduramicin
Catalase
Glutathione Peroxidase
Superoxide Dismutase