Plastics are to date considered one of the main detrimental drivers for the health of aquatic ecosystems, both in marine and inland waters. Regarding the latter habitat, it seems surprising how the plastic effects on benthic invertebrates are neglected since macroinvertebrates have a long tradition in the water quality assessment activities. In this context, we propose timely indoor observations on the exposure of caddisfly Odontocerum albicorne and mayfly Ephemera danica to various microplastic polymers (ABS, PET, PP, PS, PVDF). Three different experimental designs were performed on caddisflies and mayflies by exposing their larvae to natural and microplastic substrates. Our findings highlighted how microplastics affected both caddisflies in rebuilding its own case (after having removed the natural one) and mayflies burrowing. Particularly, all caddisflies rebuilt cases using the microplastic polymers provided instead of natural items only. Moreover, we provide the first evidence that mayflies burrow mainly in microplastic substrates rather than in natural ones. Our research highlights that macroinvertebrate larvae would use naturally occurring microplastics and this could be of particular concern in freshwaters with high contamination by plastics. Indeed, larvae appear to not necessarily perceive microplastics as a direct stressor. Further studies ought to be conducted to understand the chronic perturbation on larvae fitness and for example, on drift behaviour. Also, further investigations are needed to understand the potentialities of using plastics by benthic macroinvertebrates.