Recent environmental applications of and development prospects for immobilized laccase: a review

Abstract

Laccases have enormous potential as promising 'green' biocatalysts in environmental applications including wastewater treatment and polluted soil bioremediation. The catalytic oxidation reaction they perform uses only molecular oxygen without other cofactors, and the only product after the reaction is water. The immobilization of laccase offers several improvements such as protected activity and enhanced stability over free laccase. In addition, the reusability of immobilized laccase is adistinct advantage for future applications. This review covers the sources of and progress in laccase research, and discusses the different methodologies of laccase immobilization that have emerged in the recent 5-10 years, as well as its applications to environmental fields, and evaluates these emerging technologies. Abbreviations: (2,4,6-TCP): 2,4,6-trichlorophenol; (2,4-DCP): 2,4-dichlorophenol; (ABTS), 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid); (ACE), acetaminophen; (BC-AS), almond shell; (BC-PM), pig manure; (BC-PW), pine wood; (BPA), bisphenol A; (BPA), bisphenol A; (BPF), bisphenol F; (BPS), bisphenol S; (C₆₀), fullerene; (Ca-AIL), calcium-alginate immobilized laccase; (CBZ), carbamazepine; (CETY), cetirizine; (CHT-PGMA-PEI-Cu (II) NPs), Cu (II)-chelated chitosan nanoparticles; (CLEAs), cross-linked enzyme aggregates; (CMMC), carbon-based mesoporous magnetic composites; (COD), chemical oxygen demand; (CPH), ciprofloxacin hydrochloride; (CS), chitosan; (CTC), chlortetracycline; (Cu-AIL), copper-alginate immobilized laccase; (DBR K-4BL), Drimarene brilliant red K-4BL; (DCF), diclofenac; (E1),estrone; (E2), 17 β-estradiol; (EDC), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride; (EDCs), endocrine disrupting chemicals; (EE2), 17α-ethinylestradiol; (EFMs), electrospun fibrous membranes; (FL), free laccase; (fsMP), fumed silica microparticles; (GA-CBs), GLU-crosslinked chitosan beads; (GA-CBs), glutaraldehyde-crosslinked chitosan beads; (GA-Zr-MOF), graphene aerogel-zirconium-metal organic framework; (GLU), glutaraldehyde; (GO), graphene oxide; (HMCs), hollow mesoporous carbon spheres; (HPEI/PES), hyperbranched polyethyleneimine/polyether sulfone; (IC), indigo carmine; (IL), immobilized laccase; (k_cat), catalytic constant; (K_m), Michealis constant; (M-CLEAs), Magnetic cross-linked enzyme aggregates; (MMSNPs-CPTS-IDA-Cu²⁺), Cu²⁺-chelated magnetic mesoporous silica nanoparticles; (MSS), magnetic mesoporous silica spheres; (MWNTs), multi-walled carbon nanotubes; (MWNTs), multi-walled carbon nanotubes; (NHS), N-hydroxy succinimide; (O-MWNTs), oxidized-MWNTs; (P(AAm-NIPA)), poly(acrylamide-N-isopropylacrylamide); (p(GMA)), poly(glycidyl methacrylate); (p(HEMA)), poly(hydroxyethyl methacrylate); (p(HEMA-g-GMA)-NH₂, poly(glycidyl methacrylate) brush grafted poly(hydroxyethyl methacrylate); (PA6/CHIT), polyamide 6/chitosan; (PAC), powdered active carbon; (PAHs), polycyclic aromatic hydrocarbons; (PAM-CTS), chitosan grafted polyacrylamide hydrogel; (PAN/MMT/GO), polyacrylonitrile/montmorillonite/graphene oxide; (PAN/PVdF), polyacrylonitrile/polyvinylidene fluoride; (PEG), poly ethylene glycol; (PEI), Poly(ethyleneimine); (poly(4-VP)), poly(4-vinyl pyridine); (poly(GMA-MAA)), poly(glycidyl methacrylate-methacrylic acid); (PVA), polyvinyl alcohol; (RBBR), Remazol Brilliant Blue R; (SDE), simulated dye effluent; (semi-IPNs), semi-interpenetrating polymer networks; (TC), tetracycline; (TCH), tetracycline hydrochloride; (TCS), triclosan; (Vmax), maximum activity; (Zr-MOF, MMU), micro-mesoporous Zr-metal organic framework.

Keywords: Laccases; combination methodologies; environmental application; immobilization; prospect.