Corynebacterium anserum sp. nov., isolated from the faeces of greater white-fronted geese (Anser albifrons) at Poyang Lake, PR China

Qian Liu; Kui Wu; Guoyin Fan; Xiangning Bai; Xi Yang; Yanyu Pan; Lijiao Cao; Wentao Song; Shengen Chen; Yanwen Xiong; Haiying Chen

doi:10.1099/ijsem.0.004637

Corynebacterium anserum sp. nov., isolated from the faeces of greater white-fronted geese (Anser albifrons) at Poyang Lake, PR China

Int J Syst Evol Microbiol. 2021 Feb;71(2). doi: 10.1099/ijsem.0.004637.

Authors

Qian Liu^{1

2

3}, Kui Wu³, Guoyin Fan³, Xiangning Bai¹, Xi Yang¹, Yanyu Pan¹, Lijiao Cao¹, Wentao Song³, Shengen Chen³, Yanwen Xiong¹, Haiying Chen^{2

3}

Affiliations

¹ State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, PR China.
² Jiangxi Province Key Laboratory of Preventive Medicine, School of Public Health, Nanchang University, Nanchang 330006, PR China.
³ The Collaboration Unit for Field Epidemiology of State Key Laboratory of Infectious Disease Prevention and Control, Jiangxi Provincial Key Laboratory of Animal-origin and Vector-borne Diseases, Nanchang Center for Disease Control and Prevention, Nanchang 330038, PR China.

PMID: 33427608
DOI: 10.1099/ijsem.0.004637

Abstract

Two Gram-stain-positive, facultatively aerobic, non-motile and rod- to coccoid-shaped bacterial strains, 23H37-10^T and 4HC-13, were isolated from the faeces of greater white-fronted geese (Anser albifrons) at Poyang Lake, Jiangxi Province, PR China. Optimal growth was observed at 35-37 °C, pH 7.0-8.0 and with 0.5-1.5 % (w/v) NaCl. The 16S rRNA gene sequences of strains 23H37-10^T and 4HC-13 were identical. Phylogenetic and phylogenomic analyses indicated that strains 23H37-10^T and 4HC-13 formed an independent cluster within the genus Corynebacterium and showed 98.8, 97.4, 97.4 and 97.2 % 16S rRNA gene sequence similarity to Corynebacterium urogenitale LMM 1652^T, Corynebacterium urealyticum DSM 7109^T, Corynebacterium falsenii DSM 44353^T and Corynebacterium jeikeium NCTC 11913^T, respectively. Cells contained C_{18 :1} ω9c, C_{18 : 0} and C_{16 : 0} as the major cellular fatty acids and MK-9 (H₂) as the predominant respiratory quinone. The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidyl inositol mannosides, two unidentified phospholipids, four unidentified glycolipids and one unidentified lipid. Strain 23H37-10^T contained mycolic acids, with meso-diaminopimelic acid and arabinose as the major whole-cell hydrolysates. The genome G+C content of strains 23H37-10^T and 4HC-13 was 55.2 mol%. The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strains 23H37-10^T and 4HC-13 were 94.4 and 99.6 %, respectively. Strains 23H37-10^T and 4HC-13 had dDDH and ANI values of less than 70 and 96 % with all available genomes of the genus Corynebacterium, respectively. The differential genotypic inferences, together with phenotypic and biochemical characteristics, suggested that strains 23H37-10^T and 4HC-13 represent a novel species within the genus Corynebacterium, for which the name Corynebacterium anserum sp. nov. is proposed. The type strain is 23H37-10^T (=GDMCC 1.1737^T=KACC 21672^T).

Keywords: Anser albifrons; Corynebacterium anserum; Poyang Lake; novel species.

MeSH terms

Animals
Bacterial Typing Techniques
Base Composition
China
Corynebacterium / classification*
Corynebacterium / isolation & purification
DNA, Bacterial / genetics
Fatty Acids / chemistry
Feces / microbiology*
Geese / microbiology*
Lakes
Nucleic Acid Hybridization
Peptidoglycan / chemistry
Phospholipids / chemistry
Phylogeny*
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Vitamin K 2 / analogs & derivatives
Vitamin K 2 / chemistry

Substances

DNA, Bacterial
Fatty Acids
Peptidoglycan
Phospholipids
RNA, Ribosomal, 16S
Vitamin K 2
menaquinone 9