Biological decolorization of Amaranth dye with Trametes polyzona in an airlift reactor under three airflow regimes

R Pérez-Cadena; Y García-Esquivel; Y E Castañeda-Cisneros; M G Serna-Díaz; M R Ramírez-Vargas; C R Muro-Urista; A Téllez-Jurado

doi:10.1016/j.heliyon.2020.e05857

Biological decolorization of Amaranth dye with Trametes polyzona in an airlift reactor under three airflow regimes

Heliyon. 2020 Dec 29;6(12):e05857. doi: 10.1016/j.heliyon.2020.e05857. eCollection 2020 Dec.

Authors

R Pérez-Cadena¹, Y García-Esquivel², Y E Castañeda-Cisneros², M G Serna-Díaz³, M R Ramírez-Vargas², C R Muro-Urista⁴, A Téllez-Jurado²

Affiliations

¹ Ingeniería en Energía, Universidad Politécnica Metropolitana de Hidalgo, Boulevard Acceso a Tolcayuca No. 1009, ExHacienda de San Javier, C.P. 43860, Tolcayuca, Hidalgo, Mexico.
² Laboratorio de AgroBiotecnología, Universidad Politécnica de Pachuca, Carretera Pachuca-Cd. Sahagún, km 20, ExHacienda de Santa Bárbara, C.P. 43830, Zempoala, Hidalgo, Mexico.
³ Departamento de Ingeniería, Universidad Autónoma del Estado de Hidalgo, Carretera Pachuca-Tulancingo, km 45, Col. Carboneras, C.P. 42184, Mineral de la Reforma, Hidalgo, Mexico.
⁴ Departamento de Posgrado e Ingeniería Química, Instituto Tecnológico de Toluca, Av. Tecnológico s/n, Col. Agrícola Bellavista, C.P. 52149, Metepec, Estado de México, Mexico.

Abstract

In the present work, a strain of the basidiomycete fungus Trametes polyzona was used to decolorize the Amaranth dye. The decolorization was carried out in an Airlift reactor with three flow regimes: 1, 2, and 3 vvm. The results showed that the decolorization was a function of the flow regime. The decolorization times for the regimes of 1, 2, and 3 vvm were 30, 25, and 19 days, respectively. The COD (Chemical Oxygen Demand) decreased from 1600 to 72 mg COD/L. The enzymatic activity kinetics of laccase (Lcc), lignin peroxidase (LiP), and manganese peroxidase (MnP) were determined. In all the treatments, the enzyme LiP was expressed during the first 6 days, at which point 80% decolorization was observed, whereas Lcc and MnP enzymes were produced from day 6 until the end of the decolorization process. The effluent generated showed no inhibitory effects on the growth of the algae Nannochloropsis salina. T. polyzona showed great versatility in the decolorization of synthetic effluents containing the Amaranth dye, and the fungus was able to use this dye as its only carbon source starting at the beginning of the process. LiP was the enzyme that contributed the most to the decolorization process, and on average, 95% decreases in color and the COD were observed.

Keywords: Aeration; Amaranth dye; COD; Decolorization; Ligninolytic enzymes; Trametes polyzona.