Novel strategy for biodegradation of 4-nitrophenol by the immobilized cells of Pseudomonas sp . YPS3 with Acacia gum

Dharman Kalaimurugan; Palaniappan Sivasankar; Kaliannan Durairaj; Manokaran Lakshmanamoorthy; Sulaiman Ali Alharbi; Sulaiman A Al Yousef; Arunachalam Chinnathambi; Srinivasan Venkatesan

doi:10.1016/j.sjbs.2020.11.019

Novel strategy for biodegradation of 4-nitrophenol by the immobilized cells of Pseudomonas sp . YPS3 with Acacia gum

Saudi J Biol Sci. 2021 Jan;28(1):833-839. doi: 10.1016/j.sjbs.2020.11.019. Epub 2020 Nov 11.

Authors

Dharman Kalaimurugan¹, Palaniappan Sivasankar¹, Kaliannan Durairaj^{1

2}, Manokaran Lakshmanamoorthy¹, Sulaiman Ali Alharbi³, Sulaiman A Al Yousef⁴, Arunachalam Chinnathambi³, Srinivasan Venkatesan¹

Affiliations

¹ Department of Environmental Science, School of Life Sciences, Periyar University, Salem, Tamil Nadu, India.
² Zoonosis Research Center, Department of Infection Biology, School of Medicine, Wonkwang University, Iksan 54538, Republic of Korea.
³ Department of Botany and Microbiology, College of Science, King Saud University, PO Box-2455, Riyadh 11451, Saudi Arabia.
⁴ Department of Clinical Laboratory Sciences, College Applied Medical Science, University of Hafr Albatin, P.O. Box 1803, Hafar Al-Batin 31991, Saudi Arabia.

Abstract

The mass organic compound 4-nitrophenol with low molecular is involved in many chemicals processes and most common organic pollutants. 4-Nitrophenol (4-NP) existing in soils and water bodies, thereby causing severe environmental impact and health risk. Even low concentrations are harmful to health and potential mutagenic and carcinogenic. Though the existing methods of biodegradation though effective, their popularity is hindered due to high cost. Hence, in the present study a less expensive method involving the use of Pseudomonas sp. with gum arabic (PAA) was tested. The biodegradation of 4-NP was thoroughly investigated by progressive characterization methods. The promising Pseudomonas sp. YPS 3 was identified with biochemical and molecular identification process. The average particle sizes of stable crystalline PAA was 8-20 nm. The experiments were conducted with optimized parameters viz., pH (7.0), concentration (30 ppm), temperature (37 °C) and time (6 h). The study was tested as adsorbent particle size on 4-NP concurrent adsorption-biodegradation. In addition, these Pseudomonas sp. YPS3 and its PAA are used as an eco-friendly for removal of toxic organic 4-NP pollutant from the ecosystems.

Keywords: 4-Nitrophenol; Biodegradation; Gum arabic; Immobilized cells; Pseudomonas sp..