Comparative phenotypic characterization identifies few differences in the metabolic capacity between Escherichia coli ST131 subclones

Abdulaziz Alqasim; Ahmad Abu Jaffal; Naif Almutairi; Abdullah A Alyousef

doi:10.1016/j.sjbs.2020.11.008

Comparative phenotypic characterization identifies few differences in the metabolic capacity between Escherichia coli ST131 subclones

Saudi J Biol Sci. 2021 Jan;28(1):762-769. doi: 10.1016/j.sjbs.2020.11.008. Epub 2020 Nov 11.

Authors

Abdulaziz Alqasim¹, Ahmad Abu Jaffal², Naif Almutairi¹, Abdullah A Alyousef¹

Affiliations

¹ Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, P.O. Box 10219, Riyadh 11433, Saudi Arabia.
² Department of Clinical Laboratory Sciences, King Saud Bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia.

Abstract

Background: Extraintestinal pathogenic Escherichia coli (ExPEC) is responsible for causing many infections such as urinary tract infections (UTIs). The current dissemination of the multidrug resistant (MDR) ExPEC clone, Escherichia coli sequence type 131 (E. coli ST131), poses a real threat to public health worldwide. This study aimed to determine and compare the metabolic capacity of a collection of ExPEC isolates including ST131, non-ST131 and various ST131 subclones, and sought to assess the association between antimicrobial resistance and metabolic capacity of ST131 isolates.

Methods: The metabolic activity of forty urine E. coli isolates, collected from in-patients hospitalized at tertiary hospital in Riyadh, was tested using KB009 Hi carbohydrate kit, and then statistically analysed to assess the difference in the metabolic profiles between ST131 and non-ST131 isolates, and between ST131 subclones.

Results: The data of this study found almost similar metabolic profiles between ST131 and non-ST131, suggesting that ST131 is not a metabolically unique clone of ExPEC. There was also no link between antimicrobial susceptibility profiles and high metabolic capacity of ST131 isolates. Testing the biochemical activity of isolates belonging to ST131 subclones found higher activity of H30 subclone than non-H30 isolates, however it revealed few significant differences between these subclones.

Conclusion: This study demonstrated no difference in the metabolism of ST131 and non-ST131, although it uncovered the presence of few significant differences in the metabolic capacity between ST131 subclones. Carrying out whole-genome based studies on ST131 and its main subclones is essential to elucidate the genetic factors responsible for the success of particular ST131 subclones.

Keywords: Antimicrobial resistance; E. coli; H30; Metabolism; ST131.