The Single-Cell Gel Electrophoresis Genotoxin Sensitivity Assay

Methods Mol Biol. 2021:2240:1-12. doi: 10.1007/978-1-0716-1091-6_1.

Abstract

The single-cell gel electrophoresis-based genotoxin sensitivity assay (GSA) is an ex vivo approach which enables to study the impact of a variety of dietary factors, occupational exposures, and diseases on the sensitivity of humans towards genotoxic chemicals which cause adverse health effects such as cancer, accelerated aging, and infertility.

Keywords: DNA damage; Genotoxic carcinogens; Lymphocytes; Single-cell gel electrophoresis assay.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carcinogenicity Tests / methods*
  • Carcinogens / toxicity*
  • Cell Survival
  • Cells, Cultured
  • Comet Assay / methods*
  • DNA Damage
  • Humans
  • Lymphocytes / drug effects
  • Mutagens / toxicity*
  • Single-Cell Analysis / methods*

Substances

  • Carcinogens
  • Mutagens