Clonal integration and phosphorus management under light heterogeneity facilitate the growth and diversity of understory vegetation and soil fungal communities

Abstract

The spatial heterogeneity of light and nutrient deficiency occurs in many forest understories. Proper fertilization management of unhealthy forests can benefit forest understory diversity and improve the stability of degraded soil; and clonal integration is a major advantage of resource sharing for many forest understory vegetation, such as pteridophytes. In this study, we tested whether understory soil fertilization and clonal integration under light heterogeneity were able to increase the performance and diversity of understory vegetation and soil microbial communities in nature. Field experiments-with or without phosphorus (P) addition, with intact or severed rhizome, and under homogeneous or heterogeneous light environments-were conducted in the understory of a typical evergreen forest in southeast China. Light heterogeneity, P addition and clonal integration promoted the growth, diversity and evenness of ferns and soil microbial biomass C, N and P (MBC, MBN and MBP) at both experimental plot and patch level. They also increased Chao1 richness and Shannon diversity of soil fungal communities at patch level, especially in the high light patches with P addition. The positive effects of P addition and clonal integration on the growth and diversity of ferns and soil microbial biomass were greatly increased under heterogeneous light. The positive effects of clonal integration on the growth were the greatest in the heterogeneous high light patches. Moreover, the interactive effect of P addition and clonal integration increased soil MBN and MBP. Clonal integration promoted the increased growth and diversity of ferns and soil MBC in the heterogeneous light environment (9.35%-35.19%), and enhanced soil MBN and MBP in the P addition treatment (9.03%-12.96%). The interactive effect of P addition and clonal integration largely led to the transition of fungal groups from slow-growing oligotrophic types to fast-growing copiotrophic types. Our results show that the interactions between clonal integration and/or P addition under light heterogeneity increase the benefits of ferns in light-rich patches, and further promote integrative performance of ferns and soil microbial communities.

Keywords: Clonal growth; Diversity of fungal communities; Phosphorus deficiency; Soil management; Soil microbial biomass; Understory vegetation management.