Sodium molybdate induces heterophil extracellular traps formation in chicken

Aimin Jiang; Yong Zhang; Di Wu; Shuangqiu Li; Ziyi Liu; Zhengtao Yang; Zhengkai Wei

doi:10.1016/j.ecoenv.2020.111886

Sodium molybdate induces heterophil extracellular traps formation in chicken

Ecotoxicol Environ Saf. 2021 Mar 1:210:111886. doi: 10.1016/j.ecoenv.2020.111886. Epub 2021 Jan 8.

Authors

Aimin Jiang¹, Yong Zhang¹, Di Wu¹, Shuangqiu Li¹, Ziyi Liu¹, Zhengtao Yang², Zhengkai Wei³

Affiliations

¹ College of Life Sciences and Engineering, Foshan University, Foshan 528225, Guangdong, PR China; College of Veterinary Medicine, Jilin University, Changchun 130062, Jilin, PR China.
² College of Life Sciences and Engineering, Foshan University, Foshan 528225, Guangdong, PR China.
³ College of Life Sciences and Engineering, Foshan University, Foshan 528225, Guangdong, PR China. Electronic address: wei_zhengkai@126.com.

PMID: 33421725
DOI: 10.1016/j.ecoenv.2020.111886

Abstract

Molybdenum (Mo) is not only an important rare metal that is widely used in industrial production but also an essential trace element for plants and animals. Nevertheless, in Mo polluted areas, excess Mo intake will not only cause gout in humans but also cause diarrhea in livestock and growth inhibition of chickens. Heterophils extracellular traps (HETs) are an important way to clear pathogens in the innate immune system of the chicken. However, the effects of Mo on the innate immune responses of HETs formation in chicken, and the mechanism undergoing this phenomenon remain unknown. In the study, we firstly aim to investigate the effects of sodium molybdate (Na₂MoO₄) on chicken HETs formation in vitro, and further to explore its related metabolic requirements and molecular mechanisms. Chicken heterophils were cultured with Na₂MoO₄, and Na₂MoO₄-induced HETs structures were analyzed by confocal microscopy. Moreover, Na₂MoO₄-induced HETs were quantified by Quant-iT PicoGreen® dsDNA Assay kits and fluorescence microplate. It has been shown that Na₂MoO₄ truly triggered HETs-like structures that were composed of DNA decorated with citrullinated histone 3 (citH3) and elastase. The inhibitors of NADPH oxidase, ERK_1/2 and p38 MAPK signaling pathway significantly reduced Na₂MoO₄-induced HETs formation. Further experiments on energy metabolism involving Na₂MoO₄-induced HETs formation showed that Na₂MoO₄-induced HETs release was relevant to glucose, and the inhibitors of glycolysis including 3PO, AZD23766 and 3-Bromopyuvic acid, the inhibitors of glucose transport including STF31 and Ritonavir and NSC23766 significantly decreased Na₂MoO₄-induced HETs formation_. In summary, these results demonstrate that Mo does induce chicken HETs formation in vitro, and the formation of HETs is a process relying on glucose transport 1 (GLUT1)，glucose transport 4 (GLUT4), glycolysis, and ROS production depended on the activation of NADPH oxidase, ERK_1/2 and p38 signaling pathways, which also reflects the early innate immune responses of chicken against excessive molybdenum intake.

Keywords: Chicken; Glycolysis; Heterophils extracellular traps; ROS; Sodium molybdate.

MeSH terms

Animals
Chickens / immunology*
Extracellular Traps / drug effects*
Glucose Transporter Type 1 / immunology
Glucose Transporter Type 4 / immunology
Glycolysis
Immunity, Innate / drug effects
MAP Kinase Signaling System / drug effects
Molybdenum / toxicity*
NADPH Oxidases / immunology
Neutrophils / drug effects*
Neutrophils / immunology
Reactive Oxygen Species / immunology

Substances

Glucose Transporter Type 1
Glucose Transporter Type 4
Reactive Oxygen Species
Molybdenum
sodium molybdate(VI)
NADPH Oxidases