A novel study for joint toxicity of typical aromatic compounds in coal pyrolysis wastewater by Tetrahymena thermophile

Abstract

The coal pyrolysis wastewater (CPW) contributed to aquatic environment contamination with amount of aromatic pollutants, and the research on joint toxicity of the mixture of aromatic compounds was vital for environmental protection. By using Tetrahymena thermophile as non-target organism, the joint toxicity of typical nonpolar narcotics and polar narcotics in CPW was investigated. The results demonstrated that the nonpolar narcotics exerted chronic and reversible toxicity by hydrophobicity-based membrane perturbation, while polar narcotics performed acute toxicity by irreversible damage of cells. As the most hydrophobic nonpolar narcotics, indole and naphthalene caused the highest joint toxicity in 24 h with the lowest EC_50mix (24.93 mg/L). For phenolic compounds, the combination of p-cresol and p-nitrophenol also showed the top toxicity (EC_50mix = 10.9 mg/L) with relation to high hydrophobicity, and the joint toxicity was obviously stronger and more acute than that of nonpolar narcotics. Furthermore, by studying the joint toxicity of nonpolar narcotics and polar narcotics, the hydrophobicity-based membrane perturbation was the first step of toxicity effects, and afterwards the acute toxicity induced by electrophilic polar substituents of phenols dominated joint toxicity afterwards. This toxicity investigation was critical for understanding universal and specific effects of CPW to aquatic organisms.

Keywords: Aromatic compounds; Coal pyrolysis wastewater; Joint toxicity; Tetrahymena thermophile.