Near-infrared light II - assisted rapid biofilm elimination platform for bone implants at mild temperature

Guannan Zhang; Yongqiang Yang; Jing Shi; Xiaohong Yao; Weiyi Chen; Xiaochun Wei; Xiangyu Zhang; Paul K Chu

doi:10.1016/j.biomaterials.2020.120634

Near-infrared light II - assisted rapid biofilm elimination platform for bone implants at mild temperature

Biomaterials. 2021 Feb:269:120634. doi: 10.1016/j.biomaterials.2020.120634. Epub 2020 Dec 30.

Authors

Guannan Zhang¹, Yongqiang Yang², Jing Shi³, Xiaohong Yao¹, Weiyi Chen⁴, Xiaochun Wei⁵, Xiangyu Zhang⁶, Paul K Chu⁷

Affiliations

¹ Laboratory of Biomaterial Surfaces & Interfaces, Institute of New Carbon Materials, Taiyuan University of Technology, Taiyuan, 030024, China.
² Jiangsu Provinces Special Equipment Safety Supervision Inspection Institute, Branch of Wuxi, National Graphene Products Quality Supervision and Inspection Center, Jiangsu, Wuxi, 214174, PR China.
³ Analytical Instrumentation Center, State Key Laboratory of Coal Conversion, Institute of Coal Chemistry, Chinese Academy of Sciences, Taiyuan, 030001, China.
⁴ College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan, 030024, China.
⁵ Shanxi Key Laboratory of Bone and Soft Tissue Injury Repair, Department of Orthopedics, Second Hospital of Shanxi Medical University, Taiyuan, 030024, China.
⁶ Laboratory of Biomaterial Surfaces & Interfaces, Institute of New Carbon Materials, Taiyuan University of Technology, Taiyuan, 030024, China; College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan, 030024, China; Shanxi Key Laboratory of Bone and Soft Tissue Injury Repair, Department of Orthopedics, Second Hospital of Shanxi Medical University, Taiyuan, 030024, China. Electronic address: zhangxiangyu@tyut.edu.cn.
⁷ Department of Physics, Department of Materials Science and Engineering, And Department of Biomedical Engineering, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, China. Electronic address: paul.chu@cityu.edu.hk.

PMID: 33421708
DOI: 10.1016/j.biomaterials.2020.120634

Abstract

Light-triggered therapy is a prospective method to combat implant-associated infection but near-infrared I (NIR-I) light has insufficient penetrating ability in tissues and local hyperthermia induced by the photothermal treatment may destroy surrounding healthy tissues. Herein, a near-infrared II (NIR-II) phototherapy system composed of upconversion elements doped titanium dioxide nanorods (TiO₂ NRs)/curcumin (Cur)/hyaluronic acid (HA)/bone morphogenetic protein-2 (BMP-2) is designed for biomedical titanium and demonstrated to overcome the above hurdles simultaneously. Incorporation of F, Yb, and Ho not only improves the photocatalytic ability, but also renders the implants with the upconversion capability, so that the NRs can generate enough reactive oxygen species (ROS) when irradiated by the NIR-II laser. Furthermore, the combined actions of quorum sensing inhibitors, ROS, and physical puncture by NRs eliminate Staphylococcus aureus biofilms on titanium rapidly at a mild temperature of 45 °C by only requiring irradiation with the 1060 nm laser for only 15 min in vitro and in vivo. The presence of Cur mitigates the immune response and BMP-2 improves osteogenic differentiation, thus accelerating new bone formation. This low-temperature NIR-II light-triggered antibacterial platform has large potential in combating deep-tissue infection in clinical applications.

Keywords: Anti-Biofilm; Implants; Osseointegration; Phototherapy; Second near-infrared laser.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biofilms*
Infrared Rays
Osteogenesis*
Phototherapy
Prospective Studies
Temperature