Identification of protein/mRNA network involving the PSORS1 locus gene CCHCR1 and the PSORS4 locus gene HAX1

Cinzia Pisani; Annalisa Onori; Francesca Gabanella; Maria Grazia Di Certo; Claudio Passananti; Nicoletta Corbi

doi:10.1016/j.yexcr.2021.112471

Identification of protein/mRNA network involving the PSORS1 locus gene CCHCR1 and the PSORS4 locus gene HAX1

Exp Cell Res. 2021 Feb 15;399(2):112471. doi: 10.1016/j.yexcr.2021.112471. Epub 2021 Jan 5.

Authors

Cinzia Pisani¹, Annalisa Onori², Francesca Gabanella³, Maria Grazia Di Certo⁴, Claudio Passananti⁵, Nicoletta Corbi⁶

Affiliations

¹ CNR-Institute of Molecular Biology and Pathology, Department of Molecular Medicine, Sapienza University of Rome, Italy. Electronic address: cinzia.pisani@cnr.it.
² CNR-Institute of Molecular Biology and Pathology, Department of Molecular Medicine, Sapienza University of Rome, Italy. Electronic address: annalisa.onori@cnr.it.
³ CNR-Institute of Molecular Biology and Pathology, Department of Molecular Medicine, Sapienza University of Rome, Italy; CNR-Institute of Biochemistry and Cell Biology, Department of Sense Organs, Sapienza University of Rome, Italy. Electronic address: francesca.gabanella@gmail.com.
⁴ CNR-Institute of Biochemistry and Cell Biology, Department of Sense Organs, Sapienza University of Rome, Italy. Electronic address: mariagrazia.dicerto@cnr.it.
⁵ CNR-Institute of Molecular Biology and Pathology, Department of Molecular Medicine, Sapienza University of Rome, Italy. Electronic address: claudio.passananti@cnr.it.
⁶ CNR-Institute of Molecular Biology and Pathology, Department of Molecular Medicine, Sapienza University of Rome, Italy. Electronic address: nicoletta.corbi@cnr.it.

PMID: 33417922
DOI: 10.1016/j.yexcr.2021.112471

Abstract

CCHCR1 (Coiled-Coil alpha-Helical Rod 1), maps to chromosomal region 6p21.3, within the major psoriasis susceptibility locus PSORS1. CCHCR1 itself is a plausible psoriasis candidate gene, however its role in psoriasis pathogenesis remains unclear. We previously demonstrated that CCHCR1 protein acts as a cytoplasmic docking site for RNA polymerase II core subunit 3 (RPB3) in cycling cells, suggesting a role for CCHCR1 in vesicular trafficking between cellular compartments. Here, we report a novel interaction between CCHCR1 and the RNA binding protein HAX1. HAX1 maps to chromosomal region 1q21.3 within the PSORS4 locus and is over-expressed in psoriasis. Both CCHCR1 and HAX1 share subcellular co-localization with mitochondria, nuclei and cytoplasmic vesicles as P-bodies. By a series of ribonucleoprotein immunoprecipitation (RIP) assays, we isolated a pool of mRNAs complexed with HAX1 and/or CCHCR1 proteins. Among the mRNAs complexed with both CCHCR1 and HAX1 proteins, there are Vimentin mRNA, previously described to be bound by HAX1, and CAMP/LL37 mRNA, whose gene product is over-expressed in psoriasis.

Keywords: CCHCR1; HAX1; LL37; Psoriasis; RNA metabolism; Vesicular trafficking; Vimentin.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adaptor Proteins, Signal Transducing / genetics*
Adaptor Proteins, Signal Transducing / metabolism
Animals
Cells, Cultured
Gene Regulatory Networks* / genetics
Genetic Loci
Genetic Predisposition to Disease / genetics
HL-60 Cells
HeLa Cells
Humans
Infant, Newborn
Intracellular Signaling Peptides and Proteins / genetics*
Intracellular Signaling Peptides and Proteins / metabolism
Male
Polymorphism, Single Nucleotide
Protein Binding
Protein Interaction Maps* / genetics
Psoriasis / genetics*
RNA, Messenger / analysis
RNA, Messenger / genetics
RNA, Messenger / metabolism
Rabbits

Substances

Adaptor Proteins, Signal Transducing
CCHCR1 protein, human
HAX1 protein, human
Intracellular Signaling Peptides and Proteins
RNA, Messenger