Single-cell Sequencing in the Field of Stem Cells

Tian Chen; Jiawei Li; Yichen Jia; Jiyan Wang; Ruirui Sang; Yi Zhang; Ruiming Rong

doi:10.2174/1389202921999200624154445

Single-cell Sequencing in the Field of Stem Cells

Curr Genomics. 2020 Dec;21(8):576-584. doi: 10.2174/1389202921999200624154445.

Authors

Tian Chen¹, Jiawei Li¹, Yichen Jia¹, Jiyan Wang¹, Ruirui Sang¹, Yi Zhang¹, Ruiming Rong¹

Affiliation

¹ 1Department of Urology, Zhongshan Hospital, Fudan University, Shanghai, P.R. China; 2Shanghai Key Laboratory of Organ Transplantation, Shanghai, P.R. China; 3Biomedical Research Center, Institute for Clinical Sciences, Zhongshan Hospital, Fudan University, Shanghai, P.R. China; 4Department of Urology, Shanghai Public Health Clinical Center, Shanghai, P.R. China; 5Department of Transfusion, Zhongshan Hospital, Fudan University, Shanghai, P.R. China.

Abstract

Variation and heterogeneity between cells are the basic characteristics of stem cells. Traditional sequencing analysis methods often cover up this difference. Single-cell sequencing technology refers to the technology of high-throughput sequencing analysis of genomes at the single-cell level. It can effectively analyze cell heterogeneity and identify a small number of cell populations. With the continuous progress of cell sorting, nucleic acid extraction and other technologies, single-cell sequencing technology has also made great progress. Encouraging new discoveries have been made in stem cell research, including pluripotent stem cells, tissue-specific stem cells and cancer stem cells. In this review, we discuss the latest progress and future prospects of single-cell sequencing technology in the field of stem cells.

Keywords: RNA sequencing; Single-cell sequencing; cancer stem cell; heterogeneity; pluripotent stem cell; tissue-specific stem cell.

Publication types

Review