A Microfluidic/Microscopy-Based Platform for on-Chip Controlled Gene Expression in Mammalian Cells

Mahmoud Khazim; Elisa Pedone; Lorena Postiglione; Diego di Bernardo; Lucia Marucci

doi:10.1007/978-1-0716-1032-9_10

A Microfluidic/Microscopy-Based Platform for on-Chip Controlled Gene Expression in Mammalian Cells

Methods Mol Biol. 2021:2229:205-219. doi: 10.1007/978-1-0716-1032-9_10.

Authors

Mahmoud Khazim^#^{1

2

3}, Elisa Pedone^#^{1

2

3}, Lorena Postiglione^#^{1

2

3}, Diego di Bernardo^#⁴, Lucia Marucci^#^{5

6

7}

Affiliations

¹ Department of Engineering Mathematics, University of Bristol, Bristol, UK.
² School of Cellular and Molecular Medicine, University of Bristol, Bristol, UK.
³ BrisSynBio, Bristol, UK.
⁴ Telethon Institute of Genetics and Medicine, Pozzuoli (NA), Italy.
⁵ Department of Engineering Mathematics, University of Bristol, Bristol, UK. lucia.marucci@bristol.ac.uk.
⁶ School of Cellular and Molecular Medicine, University of Bristol, Bristol, UK. lucia.marucci@bristol.ac.uk.
⁷ BrisSynBio, Bristol, UK. lucia.marucci@bristol.ac.uk.

^# Contributed equally.

PMID: 33405224
DOI: 10.1007/978-1-0716-1032-9_10

Abstract

Applications of control engineering to mammalian cell biology have been recently implemented for precise regulation of gene expression. In this chapter, we report the main experimental and computational methodologies to implement automatic feedback control of gene expression in mammalian cells using a microfluidics/microscopy platform.

Keywords: Cell segmentation; Control algorithms; Feedback control; Mammalian cell; Microfluidics; PDMS.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Algorithms
Animals
Gene Expression*
Genetic Engineering
Humans
Lab-On-A-Chip Devices
Microfluidic Analytical Techniques / instrumentation*

Abstract

Publication types

MeSH terms

Grants and funding