Dnajb8, a target gene of SOX30, is dispensable for male fertility in mice

Fengsong Wang; Shuai Kong; Xuechun Hu; Xin Li; Bo Xu; Qiuling Yue; Kaiqiang Fu; Lan Ye; Shun Bai

doi:10.7717/peerj.10582

Dnajb8, a target gene of SOX30, is dispensable for male fertility in mice

PeerJ. 2020 Dec 21:8:e10582. doi: 10.7717/peerj.10582. eCollection 2020.

Authors

Fengsong Wang^#¹, Shuai Kong^#¹, Xuechun Hu², Xin Li³, Bo Xu⁴, Qiuling Yue⁵, Kaiqiang Fu⁶, Lan Ye³, Shun Bai⁴

Affiliations

¹ School of Life Science, Anhui Medical University, Hefei, China.
² Department of Urology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China.
³ State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, China.
⁴ Reproductive and Genetic Hospital, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China.
⁵ Reproductive Medicine Center, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, China.
⁶ College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, China.

^# Contributed equally.

Abstract

Background: The DNAJ family of molecular chaperones maintains protein homeostasis in mitotic and postmeiotic cells, especially germ cells. Recently, we found that the transcription factor SOX30 initiates transcription of Dnajb8 during late meiosis and spermiogenesis in mouse testes.

Methods: We used the CRISPR/Cas9 system to generate Dnajb8 mutant mice and analyze the phenotype of the Dnajb8 mutants.

Results: Although Dnajb8 is an evolutionarily conserved gene, it is not essential for spermatogenesis and male fertility. We provide this phenotypic information, which could prevent duplicative work by other groups.

Keywords: Dnajb8; Male fertility; Spermatogenesis.

Grants and funding

This study was supported by National Natural Science Foundation of China (No. 81901543, 81972641, 81901545, 81971333), the Fundamental Research Funds for the Central Universities (No. WK9110000063), State Key Laboratory of Reproductive Medicine (No. SKLRM-K201904) and the National Key Research and Development Project (2019YFA0802600). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.