Promoter P PSP 1-5- BnPSP-1 From Ramie (Boehmeria nivea L. Gaud.) Can Drive Phloem-Specific GUS Expression in Arabidopsis thaliana

Yunhe Wang; Yaning Bao; Yancheng Zheng; Ping'an Guo; Dingxiang Peng; Bo Wang

doi:10.3389/fgene.2020.553265

Promoter P _{PSP 1-5}- BnPSP-1 From Ramie (Boehmeria nivea L. Gaud.) Can Drive Phloem-Specific GUS Expression in Arabidopsis thaliana

Front Genet. 2020 Dec 16:11:553265. doi: 10.3389/fgene.2020.553265. eCollection 2020.

Authors

Yunhe Wang¹, Yaning Bao^{1

2}, Yancheng Zheng¹, Ping'an Guo³, Dingxiang Peng¹, Bo Wang¹

Affiliations

¹ MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
² College of Tobacco Science, University of Guizhou, Guiyang, China.
³ Hubei Key Laboratory of Edible Wild Plants Conservation and Utilization, University of Hubei Normal, Huangshi, China.

Abstract

Isolation of phloem-specific promoters is one of the basic conditions for improving the fiber development and resistance of ramie phloem using genetic engineering. In this study, we isolated a ramie endogenous promoter (named P _PSP1 -BnPSP-1) and analyzed the function of its truncated fragments in Arabidopsis. The results show that P _PSP1 -BnPSP-1 can drive the GUS reporter gene to be specifically expressed in the veins of Arabidopsis. After hormone and simulated drought treatment of the independent Arabidopsis lines carrying P _PSP1 -BnPSP-1 and its truncated fragments, only P _PSP1-5-BnPSP-1 (-600 to -1 bp region of P _PSP1 -BnPSP-1) is stably expressed and exhibits phloem specificity. Our findings suggest that P _PSP1-5-BnPSP-1 can be used as a phloem specific promoter for further research.

Keywords: GUS expression; UFW cloning; promoter; ramie [Boehmeria nivea (L.) Gaud.]; tissue-specific.