Target specific aptamer-induced self-assembly of fluorescent graphene quantum dots on palladium nanoparticles for sensitive detection of tetracycline in raw milk

Syed Rahin Ahmed; Satish Kumar; Greter A Ortega; Seshasai Srinivasan; Amin Reza Rajabzadeh

doi:10.1016/j.foodchem.2020.128893

Target specific aptamer-induced self-assembly of fluorescent graphene quantum dots on palladium nanoparticles for sensitive detection of tetracycline in raw milk

Food Chem. 2021 Jun 1:346:128893. doi: 10.1016/j.foodchem.2020.128893. Epub 2020 Dec 30.

Authors

Syed Rahin Ahmed¹, Satish Kumar¹, Greter A Ortega¹, Seshasai Srinivasan², Amin Reza Rajabzadeh³

Affiliations

¹ School of Engineering Practice and Technology, McMaster University, 1280 Main Street West Hamilton, Ontario L8S 4L7, Canada.
² School of Engineering Practice and Technology, McMaster University, 1280 Main Street West Hamilton, Ontario L8S 4L7, Canada. Electronic address: ssriniv@mcmaster.ca.
³ School of Engineering Practice and Technology, McMaster University, 1280 Main Street West Hamilton, Ontario L8S 4L7, Canada. Electronic address: rajaba@mcmaster.ca.

PMID: 33387835
DOI: 10.1016/j.foodchem.2020.128893

Abstract

The excessive use of tetracyclines (TCs), a bacteriostaticantibiotic, in food products, has led to the accumulation of TCs residues in the human body, affecting human health seriously. Therefore, the development of a highly sensitive method to detect TCs in food is of utmost importance. This study reports a novel sensing strategy using aptamer-induced fluorescence fluctuation of graphene quantum dots (GQDs) and palladium nanoparticles (Pd NPs) for the rapid and label-free detection of tetracycline with a limit of detection of 45 ng.mL^-1. A novel single-step synthesis of positively charged Pd NPs and one-step green synthesis of GQDs directly from graphite has been developed. The proposed strategy provides an efficient way to detect low traces of TCs and a new technique for the development of aptamer-based sensors.

Keywords: Aptasensor; Biosensor; Fluorescence assay; GQDs; Pd NPs; Tetracyclines detection.

MeSH terms

Animals
Aptamers, Nucleotide / chemistry*
Biosensing Techniques / methods*
Fluorescent Dyes / chemistry
Graphite / chemistry*
Humans
Limit of Detection
Metal Nanoparticles / chemistry
Milk / chemistry*
Palladium / chemistry*
Quantum Dots / chemistry*
Tetracycline / analysis*
Tetracycline / chemistry

Substances

Aptamers, Nucleotide
Fluorescent Dyes
Palladium
Graphite
Tetracycline