Protein corona components of polyethylene glycol-conjugated organosilica nanoparticles modulates macrophage uptake

Hyungjin Kim; Daniel Röth; Yasuhiro Isoe; Koichiro Hayashi; Chihiro Mochizuki; Markus Kalkum; Michihiro Nakamura

doi:10.1016/j.colsurfb.2020.111527

Protein corona components of polyethylene glycol-conjugated organosilica nanoparticles modulates macrophage uptake

Colloids Surf B Biointerfaces. 2021 Mar:199:111527. doi: 10.1016/j.colsurfb.2020.111527. Epub 2020 Dec 30.

Authors

Hyungjin Kim¹, Daniel Röth², Yasuhiro Isoe¹, Koichiro Hayashi³, Chihiro Mochizuki¹, Markus Kalkum², Michihiro Nakamura⁴

Affiliations

¹ Department of Organ Anatomy and Nanomedicine, Yamaguchi University, 1-1-1 Minami-Kogushi, Ube, 755-8505, Japan.
² Department of Molecular Imaging and Therapy, Beckman Research Institute of City of Hope, Duarte, CA, 91010, USA.
³ Department of Biomaterials, Faculty of Dental Science, Kyushu University, Fukuoka, 812-8582, Japan.
⁴ Department of Organ Anatomy and Nanomedicine, Yamaguchi University, 1-1-1 Minami-Kogushi, Ube, 755-8505, Japan. Electronic address: nakam@yamaguchi-u.ac.jp.

PMID: 33387796
DOI: 10.1016/j.colsurfb.2020.111527

Abstract

Fluorescent organosilica nanoparticles (FNP) conjugated with polyethylene glycol (PEG) of variant molecular weight (2 K, 12 K, 20 K, and 30 K) were prepared to investigate their cellular uptake by murine-derived macrophages. In a medium with FBS, the cellular uptake of FNP-PEGs was decreased as compared to a medium without FBS, indicating that protein corona on FNP-PEGs reduced cellular uptake. Bovine serum albumin (BSA) and hemoglobin (Hb) were detected as the most abundant components on all FNP-PEGs. Pre-coating of FNP-PEGs with BSA and Hb reduced the macrophage uptake in a medium without FBS, suggesting that these components might strengthen the stealth function of PEGs. Furthermore, there was more reduction in uptake of BSA- and Hb-coated FNP-PEGs from a medium with FBS than without FBS. BSA and Hb could be the stealth enhancement protein of FNP-PEGs in vitro.

Keywords: Macrophage; Organosilica nanoparticle; PEG molecular weight; Protein corona.

MeSH terms

Animals
Macrophages
Mice
Nanoparticles*
Polyethylene Glycols
Protein Corona*
Serum Albumin, Bovine

Substances

Protein Corona
Serum Albumin, Bovine
Polyethylene Glycols