Study of biochip integrated with microelectrodes modified by poly-dopamine-co-chitosan composite gel for separation, enrichment and detection of microbes in the aerosol

Abstract

As the urgent need for rapid detection of airborne microbes in a specific environment, a biochip which was integrated with the functions of enrichment and detection was designed and developed. It was composed of cover plate, copper microelectrodes modified with poly-dopamine-co-chitosan (PDA-co-CS) composite gel, sealing washer and substrate containing copper sheet electrode. The microbes were enriched due to the good ventilation efficiency and adhesion of the PDA-co-CS composite gel. The enrichment efficiency of microbes was 99.9%. The electrical impedance spectrum (EIS) test system which was composed of the copper electrodes and the copper sheet electrode were used to detect the concentrated microbes and establish the quantitative detection method of single microbe (S. aureus ATCC 6538) and mixed microbes (S. aureus ATCC 6538, E. coli JM109, and Candida albicans). It was shown that the biochip could respond to the aerosol with 1.26 × 10³ cfu/m³S. aureus ATCC 6538, which was 25 times as high as the detection limit of natural deposition method. Meanwhile, the Surface-enhanced Raman Spectrum (SERS) of different microbes were detected in-situ with the help of the silver sol. The SERS data of S. aureus, E. coli and Candida albicans had been analyzed to establish recognition model by the principal component analysis (PCA) method and the three microbes were successfully identified. It was demonstrated that the designed biochip could be applied for separation, enrichment and detection of microbes in the aerosol.

Keywords: Aerosol containing S. aureus; Aerosol containing mixed microbes; Biochip integrated with PDA-Co-CS composite gel; Identification of microbes by SERS; Quantitative detection of microbes by EIS.