Prediction of stable isotopes and fatty acids in subcutaneous fat of Iberian pigs by means of NIR: A comparison between benchtop and portable systems

María Inmaculada González-Martín; Olga Escuredo; Miriam Hernández-Jiménez; Isabel Revilla; Ana Ma Vivar-Quintana; Iván Martínez-Martín; Pedro Hernández-Ramos

doi:10.1016/j.talanta.2020.121817

Prediction of stable isotopes and fatty acids in subcutaneous fat of Iberian pigs by means of NIR: A comparison between benchtop and portable systems

Talanta. 2021 Mar 1:224:121817. doi: 10.1016/j.talanta.2020.121817. Epub 2020 Oct 29.

Authors

María Inmaculada González-Martín¹, Olga Escuredo², Miriam Hernández-Jiménez³, Isabel Revilla³, Ana Ma Vivar-Quintana³, Iván Martínez-Martín³, Pedro Hernández-Ramos⁴

Affiliations

¹ Analytical Chemistry, Nutrition and Bromatology, University of Salamanca Calle Plaza de los Caidos s/n, Salamanca, 37008, Spain. Electronic address: inmaglez@usal.es.
² Plant Biology and Soil Sciences. Faculty of Sciences, University of Vigo, As Lagoas, Ourense, 32004, Spain.
³ Food Technology, University of Salamanca Escuela Politécnica Superior de Zamora, Avenida Requejo 33, Zamora, 49022, Spain.
⁴ Graphic Expression in Engineering, University of Salamanca Escuela Politécnica Superior de Zamora, Avenida Requejo 33, Zamora, 49022, Spain.

PMID: 33379042
DOI: 10.1016/j.talanta.2020.121817

Abstract

The potential of a portable Near Infrared spectrophotometer compared with that of NIR benchtop equipment is assessed to determine the¹³C/¹²C relationship of stable isotopes and the fatty acid content. 105 samples of subcutaneous fat of Iberian pigs collected at the time of their slaughter have been analyzed. The analysis of stable isotopes and gas chromatography were the methods of reference used. The samples were analyzed without prior handling (portable and benchtop NIR) and after extracting the fat (benchtop NIR). The results show that with the portable equipment it is possible to determine δ¹³C (‰), 12 fatty acids, and 5 summations of fatty acids (SFA, MUFA, PUFA, w3, and w6), while with the benchtop NIR equipment it is possible to measure δ¹³C (‰), 16 fatty acids, and the 5 summationsof fatty acids. The correlation coefficients of the portable equipment were slightly lower than those of the NIR benchtop equipment.

Keywords: Benchtop NIR; Fatty acids; Iberian pig; Portable NIR; Subcutaneous fat; δ(13)C.

MeSH terms

Animals
Fatty Acids*
Isotopes
Subcutaneous Fat*
Swine

Substances

Fatty Acids
Isotopes