3D Imaging and Quantification of the Integrin at a Single-Cell Base on a Multisignal Nanoprobe and Synchrotron Radiation Soft X-ray Tomography Microscopy

Chunyu Zhang; Shengkun Yao; Chao Xu; Yanan Chang; Yunbing Zong; Kai Zhang; Xiangzhi Zhang; Lijuan Zhang; Chunying Chen; Yuliang Zhao; Huaidong Jiang; Xueyun Gao; Yaling Wang

doi:10.1021/acs.analchem.0c04662

3D Imaging and Quantification of the Integrin at a Single-Cell Base on a Multisignal Nanoprobe and Synchrotron Radiation Soft X-ray Tomography Microscopy

Anal Chem. 2021 Jan 26;93(3):1237-1241. doi: 10.1021/acs.analchem.0c04662. Epub 2020 Dec 30.

Authors

Chunyu Zhang^{1

2}, Shengkun Yao^{3

4}, Chao Xu⁵, Yanan Chang⁶, Yunbing Zong³, Kai Zhang⁷, Xiangzhi Zhang⁸, Lijuan Zhang⁸, Chunying Chen¹, Yuliang Zhao¹, Huaidong Jiang³, Xueyun Gao², Yaling Wang¹

Affiliations

¹ CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety and CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology of China, Beijing 100090, China.
² Department of Chemistry and Chemical Engineering, Beijing University of Technology, Beijing 100124, China.
³ School of Physical Science and Technology, Shanghai Tech University, Shanghai 201210, China.
⁴ Shandong Provincial Engineering and Technical Center of Light Manipulations & Shandong Provincial Key Laboratory of Optics and Photonic Device, School of Physics and Electronics, Shandong Normal University, Jinan 250358, China.
⁵ College of Chemistry and Material Science, Shandong Agricultural University, Tai'an 271018, China.
⁶ CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China.
⁷ Beijing Synchrotron Radiation Facility, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China.
⁸ Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.

PMID: 33378151
DOI: 10.1021/acs.analchem.0c04662

Abstract

The development of three-dimensional (3D) single-cell imaging and protein quantitative methods can provide more comprehensive information for diagnoses. We report the design and synthesis of a multisignal nanoprobe (AuGd_NC@BSA-CV) for single-cell 3D imaging and quantifying the integrin α_IIbβ₃ using correlated synchrotron radiation soft X-ray tomography microscopy and an iterative tomographic algorithm termed equally sloped tomography for the first time. Moreover, on the basis of the Au or Gd content of our nanoprobe, the number of integrin α_IIbβ₃ on a single cell also can be accurately quantified (1.5 × 10⁷ per cell) via inductively coupled plasma mass spectrometry.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line, Tumor
Gadolinium / chemistry
Gold / chemistry
Humans
Imaging, Three-Dimensional*
Integrins / analysis*
Nanoparticles / chemistry*
Serum Albumin, Bovine / chemistry
Single-Cell Analysis*
Synchrotrons
Tomography, X-Ray Computed*

Substances

Integrins
Serum Albumin, Bovine
Gold
Gadolinium