Protocol to Induce Wounding and Measure Membrane Repair in Caenorhabditis elegans Epidermis

Chandra Sugiarto Wijaya; Xinan Meng; Qingxian Yang; Suhong Xu

doi:10.1016/j.xpro.2020.100175

Protocol to Induce Wounding and Measure Membrane Repair in Caenorhabditis elegans Epidermis

STAR Protoc. 2020 Nov 21;1(3):100175. doi: 10.1016/j.xpro.2020.100175. eCollection 2020 Dec 18.

Authors

Chandra Sugiarto Wijaya^{1

2}, Xinan Meng^{1

2}, Qingxian Yang^{1

2}, Suhong Xu^{1

2

3}

Affiliations

¹ Center for Stem Cell and Regenerative Medicine and Department of Cardiology of The Second Affiliated Hospital, Zhejiang University School of Medicine, 310058 Hangzhou, China.
² School of Basic Medical Sciences, Zhejiang University School of Medicine, 310058 Hangzhou, China.
³ The Zhejiang University-University of Edinburgh Institute, 718 East Haizhou Rd, Haining, Zhejiang 314400, China.

Abstract

Efficient membrane repair after injury is essential for cell and animal survival. Caenorhabditis elegans epidermal cell hpy7 has emerged as a powerful genetic system to investigate the molecular mechanism of membrane repair in vivo. This protocol describes detailed approaches for how to perform wounding on the epidermis and how to examine membrane repair by trypan blue staining, confocal imaging, and data analysis. For details on the use and execution of this protocol, please refer to Meng et al. (2020).

Keywords: Cell Biology; Cell Membrane; Model Organisms.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Caenorhabditis elegans / physiology*
Cytological Techniques / methods*
Epidermis / pathology*
Green Fluorescent Proteins / metabolism
Membranes
Microscopy, Confocal
Staining and Labeling
Time-Lapse Imaging
Wound Healing*

Substances

Green Fluorescent Proteins