An Optimized Protocol of Laser Capture Microdissection for Tissue-Specific RNA Profiling in a Radish Tap Root

STAR Protoc. 2020 Sep 17;1(3):100110. doi: 10.1016/j.xpro.2020.100110. eCollection 2020 Dec 18.

Abstract

Laser capture microdissection (LCM) coupled with transcriptome profiling is a powerful technique that allows for tissue-specific gene expression analysis in a complex system. One major challenge in using this technique is to obtain RNA without compromising its integrity. Here, we present a protocol optimized for radish root tissue sections using Steedman's wax embedding to obtain high-quality RNA suitable for next-generation sequencing analysis. For complete details on the use and execution of this protocol, please refer to Hoang et al. (2020).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Gene Expression / genetics
  • Gene Expression Profiling / methods*
  • High-Throughput Nucleotide Sequencing / methods
  • Laser Capture Microdissection / methods*
  • Organ Specificity / genetics
  • Plant Roots / genetics
  • RNA / genetics
  • Raphanus / genetics*
  • Transcriptome / genetics

Substances

  • RNA