Contributions of the biochemical factors and bacterial community to the humification process of in situ large-scale aerobic composting

Xuanqing Wang; Mengmeng Wang; Juan Zhang; Zhijian Kong; Xiaosong Wang; Dongyang Liu; Qirong Shen

doi:10.1016/j.biortech.2020.124599

Contributions of the biochemical factors and bacterial community to the humification process of in situ large-scale aerobic composting

Bioresour Technol. 2021 Mar:323:124599. doi: 10.1016/j.biortech.2020.124599. Epub 2020 Dec 23.

Authors

Xuanqing Wang¹, Mengmeng Wang¹, Juan Zhang², Zhijian Kong¹, Xiaosong Wang¹, Dongyang Liu³, Qirong Shen¹

Affiliations

¹ Jiangsu Provincial Key Lab of Solid Organic Waste Utilization, Jiangsu Collaborative Innovation Center of Solid Organic Wastes, Educational Ministry Engineering Center of Resource-saving Fertilizers, Nanjing Agricultural University, Nanjing 210095, Jiangsu, People's Republic of China.
² Shandong Institute for Product Quality Inspection, People's Republic of China.
³ Jiangsu Provincial Key Lab of Solid Organic Waste Utilization, Jiangsu Collaborative Innovation Center of Solid Organic Wastes, Educational Ministry Engineering Center of Resource-saving Fertilizers, Nanjing Agricultural University, Nanjing 210095, Jiangsu, People's Republic of China. Electronic address: liudongyang@njau.edu.cn.

PMID: 33373802
DOI: 10.1016/j.biortech.2020.124599

Abstract

Multiple types of biochemical parameters were determined in the course of the composting process with rice straw and Chinese traditional medicine residues as substrates. The water-soluble fractions (WSFs) were analyzed by excitation-emission-matrix fluorescence (EEM-FL), and the maximum P_V/III value (1.2) was observed in thermophilic phase (THP). Bacterial community analysis results indicated that the genera with the capacity of degrading lignocellulose dominated in mesophilic phase (MEP) and THP. The metabolic pathways based on KEGG analysis revealed that the amino acid, carbohydrate and energy metabolism pathways in THP were higher than the other two phases. The correlation analysis between EEM-FL and the bacterial community revealed that the genera with high abundances in the THP were significantly positively correlated with fulvic acid-like materials and humic acid-like organics. The quantification results of the lignocellulose-degrading genes in different phases further verified the key functional bacteria obtained by correlation analysis during the composting process.

Keywords: Agricultural wastes; Bacterial community; Biodegradation; Composting; Enzymatic activity.

MeSH terms

Bacteria / genetics
Composting*
Humic Substances / analysis
Manure
Soil

Substances

Humic Substances
Manure
Soil