We found that changes in the concentrations of tryptophan metabolites in the blood serum and in the intestinal contents are one of the mechanisms for the formation of metabolic coupling in the system "macroorganism-intestinal microbiota", which undergoes significant changes in the development of obesity. Although blood kynurenine remained basically unchanged in obese children we found an increase in some of its serum metabolites: anthranilic, kynurenic and xanthurenic acids. It is noteworthy that in the analysis of fecal matter in obese children, revealed a 2-fold increase in the level of kynurenine while the concentration of kynurenine pathway metabolites corresponded to the level of the group of healthy children. This may indicate the metabolic activation of the microbiota associated with the intestinal mucosa. This is also supported by the absence of statistically significant differences in the concentration of indole in healthy children and in obese children in fecal analyses, and a significant increase in the concentration of indole-3-lactate and indole-3-acetate in the blood serum of obese children.
Nami ustanovleno, chto izmeneniia kontsentratsiĭ metabolitov obmena triptofana v syvorotke krovi i v soderzhimom kishechnika iavliaiutsia odnim iz mekhanizmov formirovaniia metabolicheskogo sopriazheniia v sisteme “makroorganizm-mikrobiota kishechnika”, kotoryĭ preterpevaet znachitel'nye izmeneniia pri razvitii ozhireniia. U deteĭ s ozhireniem net statisticheski znachimogo povysheniia urovnia kinurenina v syvorotke krovi, no pri étom proiskhodit povyshenie nekotorykh ego syvorotochnykh metabolitov: antranilovoĭ, kinurenovoĭ i ksanturenovoĭ kislot. Pri analize kala u deteĭ s ozhireniem vyiavlen povyshennyĭ v dva raza uroven' kinurenina. Poskol'ku kontsentratsiia metabolitov kinureninovogo puti sootvetstvuet urovniu gruppy zdorovykh deteĭ, éto mozhet svidetel'stvovat' o metabolicheskoĭ aktivatsii mikrobioty assotsiirovannoĭ so slizistoĭ obolochkoĭ kishechnika. Otsutstvie statisticheski znachimykh otlichiĭ dlia kontsentratsii indola u zdorovykh deteĭ i u deteĭ s ozhireniem v analizakh kala i znachimoe uvelichenie kontsentratsii indol-3-laktata i indol-3-atsetata v syvorotke krovi u deteĭ s ozhireniem tak zhe svidetel'stvuet v pol'zu étogo predpolozheniia.
Keywords: indoles; kynurenine; metabolic coupling; microbiota; obesity; tryptophan.