Generation of two human control iPS cell lines (UCLi016-A and UCLi017-A) from healthy donors with no known ocular conditions

Cécile Méjécase; Philippa Harding; Hajrah Sarkar; Jonathan Eintracht; Dulce Lima Cunha; Lyes Toualbi; Mariya Moosajee

doi:10.1016/j.scr.2020.102113

Generation of two human control iPS cell lines (UCLi016-A and UCLi017-A) from healthy donors with no known ocular conditions

Stem Cell Res. 2020 Dec:49:102113. doi: 10.1016/j.scr.2020.102113. Epub 2020 Dec 8.

Authors

Cécile Méjécase¹, Philippa Harding², Hajrah Sarkar¹, Jonathan Eintracht², Dulce Lima Cunha², Lyes Toualbi¹, Mariya Moosajee³

Affiliations

¹ UCL Institute of Ophthalmology, London, UK; The Francis Crick Institute, London, UK.
² UCL Institute of Ophthalmology, London, UK.
³ UCL Institute of Ophthalmology, London, UK; The Francis Crick Institute, London, UK; Moorfields Eye Hospital NHS Foundation Trust, London, UK; Great Ormond Street Hospital for Children NHS Foundation Trust, London, UK. Electronic address: m.moosajee@ucl.ac.uk.

Abstract

Two human induced pluripotent stem cell (hiPSC) lines (UCLi016-A and UCLi017-A) were generated from fibroblast cells of 23- and 34-year-old healthy male donors with no known ocular conditions. Fibroblast cells were derived from skin biopsies and reprogrammed using integration free episomal reprogramming. The established iPSC lines were found to express pluripotency markers, exhibit differentiation potential in vitro and display a normal karyotype. These cell lines will act as a control lines for researchers studying ocular diseases.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Cell Differentiation
Cell Line
Cellular Reprogramming
Fibroblasts
Humans
Induced Pluripotent Stem Cells*
Male
Skin
Young Adult

Abstract

Publication types

MeSH terms

Grants and funding